Protein Purification Scientist

Vyriad•Rochester, MN

31d•$78,000 - $102,000

About The Position

The Protein Purification Scientist supports the development and optimization of protein purification processes. This role will involve working closely with cross-functional teams to purify and characterize proteins for therapeutic and research applications.

Requirements

Bachelor's, Master's, or Ph.D. in Biochemistry, Chemical / Protein Engineering, or a related field; with hands-on experience with protein expression and purification (Mammalian & E. Coli).
3+ years of relevant experience and hands-on experience in protein purification (industry experience preferred), including Protein A, Protein G, Protein L, Ni-NTA, StrepTactin, C-tag.
Proficiency in chromatography techniques and AKTA systems.
Strong analytical skills and experience with protein characterization methods. Proficiency in biophysical and analytical methods: SPR/BLI, CD, SEC-MALS, DSF, SDS-PAGE, and Western blotting.
Excellent problem-solving abilities and attention to detail.
Ability to work independently and collaboratively in a fast-paced environment.
Strong communication and organizational skills.
Comfortable working in a fast-paced, interdisciplinary team
Strong communication skills and ability to present data and proposals to a range of audiences

Nice To Haves

Good understanding of molecular and cellular biology preferred.
Strong understanding of the biophysics of protein structure/function relationships and confidence with structure modeling and visualization tools (e.g., PyMOL, ChimeraX, AlphaFold) preferred.
Experience with scale-up and tech transfer of purification processes preferred.
Familiarity with automation and high-throughput purification systems preferred.

Responsibilities

Develop, optimize, and scale-up protein purification processes for recombinant proteins - predominantly expressed in mammalian cells.
Operate and maintain AKTA FPLC systems and other purification equipment. Perform chromatography techniques, including affinity, ion exchange, size exclusion, and hydrophobic interaction chromatography.
Analyze protein purity and quality by SDS-PAGE, Western blot, UV/Vis spectroscopy, and other analytical methods.
Characterize proteins using biophysical and biochemical methods, such as binding kinetics (BLI), thermal stability (DSF/SLS), and secondary structure (CD).
Troubleshoot and resolve technical challenges in protein purification workflows.
Manage a catalog of protein reagents, defining and maintaining quality control and release criteria, as well as generating Certificates of Analysis. Document experimental procedures, results, and findings in detailed reports and presentations.
Collaborate with upstream and downstream teams to ensure seamless process integration, to align project objectives, timelines, and design needs with downstream applications
Oversee quality control and release criteria, generate Certificates of Analysis, and manage the shipment of protein materials to internal and external partners
Stay current with advancements in protein purification technologies and methodologies. - Ensure compliance with safety protocols and maintain a clean and organized laboratory environment.
Additional duties as assigned.