Post Doctoral Fellow-MSH-12908-019
About The Position
Description Details of Research Project : Title: Hippo pathway signaling in podocyte injury and glomerular disease Our lab has identified Hippo pathway upstream regulator KIBRA (Kidney BRAin) protein as a novel mediator of podocyte injury via inhibition of downstream pro-survival transcriptional co-activator YAP. Our previous work has demonstrated that upregulated KIBRA podocyte expression increases susceptibility to a murine model of FSGS and also that WWC1 (the gene encoding KIBRA) is a risk gene for glomerular disease progression in human podocytopathies. Preliminary data suggests that increased KIBRA/Hippo pathway activation enhances podocyte injury in a diabetic milieu. We aim to test this hypothesis and the potential therapeutic role of small molecule Hippo mediators using complementary models including immortalized podocytes, hIPSC-derived podocytes and kidney organoids, diabetic rodent models, and banked human kidney tissue/biosamples. Other aims of this work include defining positive and negative regulators of WWC1/ KIBRA expression and downstream consequences of increased Hippo pathway activity on key podocyte cytostructural elements, namely the actin cytoskeleton and focal adhesion complexes.
Requirements
- Bachelors and Masters or Doctoral Degree
- at least 2 years in lab environment
Responsibilities
- Leading laboratory projects
- Preparing figures for manuscripts and writing Results sections of manuscripts
- Training junior lab members/students
- Aseptic cell culture techniques
- Protein extraction, western blotting
- RNA extraction, PCR/qPCR
- Immunofluorescence/immunohistochemistry staining
- Confocal and TIRF microscopy
- Rodent animal handling, urine collection, and intraperitoneal/retroorbital injection
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What This Job Offers
Number of Employees
5,001-10,000 employees
