Laboratory Supervisor I, Pisu Lab
About The Position
An exciting opportunity is available in the Pisu Lab at the Tulane National Biomedical Research Center for a Laboratory Supervisor I to lead hands-on experimental research on Mycobacterium tuberculosis infection and macrophage biology. This position serves as the senior technical expert in a growing program focused on defining how macrophage subsets influence host-pathogen interactions and vaccine responses. The Laboratory Supervisor I will independently perform advanced molecular, cellular, and microbiological experiments, including cloning, CRISPR/Cas9 gene editing, RNA/NGS library preparation, flow cytometry, confocal and fluorescence microscopy, and protein assays (Western blot, ELISA, Olink). The role includes macrophage culture, bacterial and viral work under BSL-2 and BSL-3 containment, and murine infection studies in close coordination with the PI. The successful candidate will generate publication-quality data, assist in manuscript and grant preparation, and train new personnel in molecular and microbiological techniques. Experience with mouse handling, breeding, and generation of knockout or Cre-lox strains is preferred. This is a fully research-oriented position suited for candidates seeking to apply deep experimental expertise in an infectious-disease and immunology context.
Requirements
- Demonstrated proficiency in aseptic technique, handling of infectious materials, and in vitro cultivation of mammalian cells, including macrophages.
- Experience with molecular and microbiological assays such as cloning, PCR/qPCR, CRISPR/Cas9 gene editing, and protein quantification (Western blot, ELISA, Olink).
- Familiarity with immunological and cell-based assays, including flow cytometry, cell sorting, and microscopy (fluorescence and confocal).
- Experience in bacterial culture and maintenance under BSL2 and/or BSL3 containment.
- Knowledge of animal infection models, including mouse handling, infection, and tissue collection under IACUC-approved protocols.
- Competence with data acquisition and analysis software (GraphPad Prism, FlowJo, R, or equivalent).
- Strong organizational, documentation, and communication skills. Ability to follow complex experimental protocols and maintain accurate records.
- Ability to complete and pass successfully the required occupational health screening referenced in the University's Animal Handler Health Surveillance Program on an annual basis.
- Bachelor's Degree in Biological Science or equivalent and three years of experience in a biomedical research facility
- M.S. Degree in Microbiology, Immunology, Molecular Biology, or a related field.
- 5+ years of hands-on experience in a biomedical research laboratory, preferably in tuberculosis or infectious disease research.
- Demonstrated proficiency with BSL3-level work and compliance with biosafety and animal protocols.
Nice To Haves
- Experience with mouse handling, breeding, and generation of knockout or Cre-lox strains.
- Experience with murine work, including breeding, colony management, and generation of knockout or Cre-lox strains.
- Prior experience with dual RNA-seq, NGS library preparation, or other high-throughput molecular assays.
Responsibilities
- Independently perform advanced molecular, cellular, and microbiological experiments, including cloning, CRISPR/Cas9 gene editing, RNA/NGS library preparation, flow cytometry, confocal and fluorescence microscopy, and protein assays (Western blot, ELISA, Olink)
- Macrophage culture, bacterial and viral work under BSL-2 and BSL-3 containment
- Murine infection studies in close coordination with the PI
- Generate publication-quality data
- Assist in manuscript and grant preparation
- Train new personnel in molecular and microbiological techniques
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What This Job Offers
Job Type
Full-time
Career Level
Mid Level
Industry
Educational Services
Number of Employees
1,001-5,000 employees
