Entry Research Services Laboratory Sciences Professional
About The Position
This position will support the laboratory of Linda Barlow at the University of Colorado School of Medicine. Our lab investigates the function of adult stem cells in maintaining the taste system, and the mechanisms of taste disruption associated with chemo- and radiotherapy. The successful candidate will assist with a project aiming to understand the cellular and molecular mechanisms of chemotherapy-induced taste disruption. The hired candidate will perform in vitro culture of taste organoids from both human and mouse tissue and perform experiments involving laboratory mice. The work will require but is not limited to generation of organoids from isolated tissues or cells, sterile culture of cells and organoids, maintenance of mouse colonies, collection of mouse tissue, cryosectioning, immunohistochemistry, gene expression analysis by qRT-PCR and Hybridization Chain Reaction (HCR), fluorescence and confocal microscopy, data collection, entry and analysis, and careful record keeping. The Research Entry Professional is expected to work in cooperation with lab members and the lab manager, Jennifer Scott, on a daily basis, and perform several aspects of lab maintenance which may include: Biohazardous, hazardous and sharps waste disposal; receiving and organizing of lab supplies and reagents; maintain lab organization and cleanliness with the help of lab members.
Requirements
- Bachelor’s degree in biology, molecular biology, chemistry, genetics, or related field.
- Applicants must meet minimum qualifications at the time of hire.
- Knowledge of laboratory techniques including safety procedures and techniques.
- Ability to communicate effectively, both in writing and orally.
- Ability to interpret and master complex research protocol information.
- Basic molecular biology and cell biology knowledge.
- Understanding of basic genetics.
- Excellent record keeping and organizational skills.
- Ability to work independently.
Nice To Haves
- One year of tissue culture experience either during or following undergraduate training.
- Background knowledge and experience with cell culture are preferred.
- Background knowledge and experience with RNA processing for qRT-PCR, immunohistochemistry and cryosectioning are highly desired.
Responsibilities
- generation of organoids from isolated tissues or cells
- sterile culture of cells and organoids
- maintenance of mouse colonies
- collection of mouse tissue
- cryosectioning
- immunohistochemistry
- gene expression analysis by qRT-PCR and Hybridization Chain Reaction (HCR)
- fluorescence and confocal microscopy
- data collection, entry and analysis
- careful record keeping
- Biohazardous, hazardous and sharps waste disposal
- receiving and organizing of lab supplies and reagents
- maintain lab organization and cleanliness with the help of lab members
Benefits
- Medical: Multiple plan options
- Dental: Multiple plan options
- Additional Insurance: Disability, Life, Vision
- Retirement 401(a) Plan: Employer contributes 10% of your gross pay
- Vacation Days: 22/year (maximum accrual 352 hours)
- Sick Days: 15/year (unlimited maximum accrual)
- Holiday Days: 15/year
- Tuition Benefit: Employees have access to this benefit on all CU campuses
- ECO Pass: Reduced rate RTD Bus and light rail service
Stand Out From the Crowd
Upload your resume and get instant feedback on how well it matches this job.
What This Job Offers
Job Type
Full-time
Career Level
Entry Level
