Entry Laboratory Science Professional

University of Colorado•Aurora, CO

4d•Onsite

About The Position

A position as an Entry Laboratory Science Professional is available within the Organoid and Tissue Modeling Shared Resource (OTMSR) at the University of Colorado Anschutz. This is a full-time (1.0 FTE) position. The Organoid and Tissue Modeling Shared Resource (OTMSR) provides cutting-edge services and expertise in in vitro 3D tissue culture modeling to researchers across the CU Anschutz campus. In this role, you will work with human 3D organoids, CRISPR/Cas9 gene editing tools, and engineered stem cell lines. Daily responsibilities will include the generation and culture of organoid cell lines, the execution and optimization of experimental protocols, and the use of advanced imaging and molecular tools.

Requirements

Bachelor’s degree in biology, molecular biology, chemistry, genetics, or a related field.
Applicants must meet minimum qualifications at the time of hire.
Applicants must be legally authorized to work in the United States without requiring sponsorship.
Knowledge of basic laboratory techniques, including safety procedures.
Able to work with mouse models (handling, husbandry, surgery, sacrificing, and dissection).
Demonstrated organizational skills (detailed record keeping, database management, etc.).
Demonstrated communication skills (data assembly and presentation, report writing, scientific conferences, etc.).
Detail-oriented, willing to learn, and motivated to troubleshoot technical problems.
Able to work in a team environment and/or independently following training.
Able to manage multiple simultaneous projects.
Able to use Microsoft Office Suite and statistical software to collect and present data.

Nice To Haves

Master’s degree in biology, molecular biology, chemistry, genetics, or a related field.
Experience with aseptic technique and culture of cell lines, primary cells, pluripotent stem cells, and/or 3D organoids.
Experience with cellular and molecular biology techniques (DNA/RNA isolation, PCR/qPCR, electrophoresis, molecular cloning, immunostaining, FACS, etc.).
Experience with microscopy and/or fluorescence imaging.
Experience with CRISPR/Cas9 based gene editing.

Responsibilities

Generation, maintenance, characterization, and biobanking of organoids produced from mouse and human-derived tissues.
Culture and differentiation of human pluripotent stem cells.
Genetic manipulation of pluripotent stem cells, including CRISPR/Cas9 gene editing (point mutation, knock-in, knock-out).
Molecular biology (PCR, electrophoresis, plasmid restriction/ligation, bacterial transformation/culture/purification).
Execution of cell-based assays - clonogenic assays, multi-color fluorescence, 3D, live cell, and confocal imaging.
Gene expression analysis (qPCR, RNAseq, scRNAseq, flow cytometry analysis, and sorting).
Maintain mouse colonies (husbandry, genotyping, tissue processing, etc.).
Development of new techniques/assays associated with organoid and iPSC culture systems.
Will occasionally assist in the Molecular Core of the Diabetes Research Center (Sanger sequencing, mycoplasma testing, DNA purification, cell line authentication, etc.).
Able to work independently and closely with a team.
Perform general laboratory maintenance, including ordering supplies, receiving laboratory reagents, making stock solutions, cleaning/maintaining laboratory space and equipment, etc.