Biologist

Axle•Baltimore, MD

4h•Onsite

About The Position

Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, they focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. Axle works with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH). Axle is seeking a Biologist to join their vibrant team at the National Institutes of Health (NIH) supporting the National Institute on Aging (NIA) located in Baltimore, MD. The primary objective of this position is to provide research services and deliverables through performance of support services for the Confocal Imaging Facility core of the NIA IRP.

Requirements

Master’s degree in Biology or a related discipline
Strong communications skills, both oral and written
Proficiency in Confocal Light Microscopy
4 years demonstrated experience in a biological research setting with routine classic embedding and sample preparation techniques including ultramicrotomy for electron microscopy methods
Demonstrated 2-5 years of experience using Scanning Electron Microscopes (SEMs) or Transmission Electron microscopes (TEMs)
Proficiency in Confocal Microscopy image acquisition and analysis
Proficiency in Immunohistochemistry IHC and ICC and immunofluorescence IF techniques
Functional knowledge of super-resolution microscopy techniques
MS office suite
FIJI-Image J
Zeiss Zen

Nice To Haves

Experience with cryo-fixation preparation techniques for cryo-electron microscopy
Experience with Focused Ion Beam – Scanning Electron Microscopy or other volumetric electron microscopy techniques
Familiarity with automated imaging systems with either light or electron microscopes in a biological research laboratory
Experience with digital imaging of biological systems using light microscopy
Familiarity with workflow using 3D reconstruction software

Responsibilities

Conduct wet-lab works including harvesting and collecting mouse embryos, tissues and organs
Perform histological techniques
Conduct various molecular biological analyses
Maintain the laboratory’s supplies, equipment and experimental records
Clean Equipment
Run Validation
Inspect Equipment
Meet with lab members to present updates
Train and supervise researchers in confocal microscopy
Perform Confocal Microscopy image acquisition and analysis
Prepare samples for transmission electron microscopy (TEM); fixation, resin embedding, ultrathin sectioning, post staining, immunogold labeling and negative staining
Prepare solutions and chemicals
Perform Immunohistochemistry (IHC), Immunocytochemistry (ICC), and immunofluorescence (IF) techniques
Provide technical experience needed to assist in studying the basic principles of plant and animal life
Study basic principles of plant and animal life, such as origin, relationship, development, anatomy, and function
Identify, classify, and study structure, behavior, ecology, physiology, nutrition, culture, and distribution of plant and animal species
Assist with performing basic laboratory experiments and procedures
Troubleshoot and optimize laboratory protocols and instrumentation
Provide user training and support
Prepare, organize and maintain various lab samples, supplies, and equipment
Operate laboratory equipment to analyze samples
Analyze laboratory findings
Operate the TEM and support equipment
Maintain a clean and organized laboratory
Learn new laboratory techniques and procedures
Program and use computers to store, process, and analyze data
Interpret research or operational data
Record research or operational data
Pursue the research goals of the laboratory by conducting wet-lab works
Work with staff on performing various experiments and procedures
Harvest and collect mouse embryos, tissues and organs
Perform histological techniques, including tissue and/or whole-mount in situ hybridization and immunohistochemistry
Prepare and organize various lab samples
Conduct molecular biological analyses
Provide a broad range of technical assistance to laboratory members
Maintain laboratory supplies, equipment and experimental records
Provide support for ordering reagents and equipment required for the laboratory