Biologist

Axle•Baltimore, MD

2h•Onsite

About The Position

Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. They work with top research organizations and facilities, including multiple institutes at the National Institutes of Health (NIH). Axle is seeking a Part-Time Biologist to join their vibrant team at the National Institutes of Health (NIH) supporting the National Institute on Aging (NIA) located in Baltimore, MD. The primary objective of this position is to provide support services to satisfy the overall operational objectives of the National Institute on Aging, delivering services and deliverables through performance of support services.

Requirements

Bachelor’s degree in Biology, Life Sciences or a related discipline.
Proficiency in MS office suite.
Ability to communicate both orally and in writing.

Responsibilities

Cultivate, maintain, expand, and freeze ES and iPS cell lines from mouse and human.
Prepare ES and iPS cells for N-linked glycoprotein labeling and analysis by Mass Spectrometry and for flow analyses, including the labeling of the cells with appropriate antibodies.
Generate primary cultures of cells for the generation of new iPS cell lines.
Characterize new iPS cell lines using appropriate techniques (morphology, staining, growth traits).
Prepare primary cell cultures suitable for iPS cell formation using standard nucleofection based techniques.
Perform primary cell cultures of mitotically inactivated feeder layers.
Design and execute flow cytometry (FACS) preps, proliferation assay, and cell surface receptor analysis.
Investigate the state of pluripotency of ES and new iPS cell lines.
Characterize nuclear proteins and cell surface proteins by western blot, immunoprecipitation, immunochemistry, and PCR.
Design primers as needed for cell characterization.
Apply a wide range of conventional, established approaches, methods, techniques, and solutions to new situations.
Identify and recommend resolution of discrepancies; recommend/determine appropriate data to use, record, or report.
Program and use computers to store, process, and analyze data.
Interpret research or operational data.
Record research or operational data.
Collect and analyze biological data about relationships among and between organisms and their environment.
Monitor and observe experiments, recording production and test data for evaluation by research personnel.
Provide technical experience needed to assist in studying the basic principles of plant and animal life.
Study basic principles of plant and animal life, such as origin, relationship, development, anatomy, and function.
Identify, classify, and study structure, behavior, ecology, physiology, nutrition, culture, and distribution of plant and animal species.
Assist with performing basic laboratory experiments and procedures.
Analyze experimental data and interpret results to write reports and summaries of findings.
Prepare, organize, and maintain various lab samples, supplies, and equipment.
Perform routine maintenance on laboratory instruments and equipment.
Ensure scientific supplies are kept stocked.
Obtain quotes and prepare purchase requests for supplies, instruments, and equipment, and submit to primary investigator and/or lab manager for approval.
Clean equipment.
Inspect equipment.
Maintain an accurate research tracking system and database for research activities; keep accurate records of daily experiments and results including research documentation.
Prepare data, results, reports, Excel spreadsheets, and PowerPoint slides for oral presentation and/or publication in journals.
Write and edit scientific papers.
Attend monthly laboratory meetings; participate in lab/unit/section meetings by presenting ongoing research and findings.
Present paper/abstract/poster at scientific meeting(s)/conference(s).
Operate laboratory instruments and equipment.
Learn new laboratory techniques and procedures.
Meet with lab members to present updates.