Biologist - BSL-3 Experience Required

Axle•Rockville, MD

About The Position

Axle is looking to add a driven, flexible, organized, and meticulous Biologist to our dynamic team. The Contractor(s) shall provide services in support of the overall functions of the Laboratory of Infectious Diseases (LID), Division of Intramural Research (DIR).

Requirements

Bachelor’s or Master’s Degree with two years’ research experience in a relevant field, with a major molecular biology, virology, or immunology component
Experience working in BSL-3 with PPAR respiratory protection
Experience with antibody titration and characterization assays

Nice To Haves

Experience with high-throughput immunology/virology screening assays preferred

Responsibilities

Assist with the development of next-generation universal influenza and betacoronavirus vaccines, and antiviral therapeutics to treat respiratory disease, including 1918 H1N1, highly pathogenic avian, and zoonotic influenza infections.
Use clinical and animal model samples to perform antibody titration and epitope analysis, including hemagglutination inhibition (HAI), neuraminidase inhibition (NAI), and enzyme-linked immunosorbent assasy (ELISA), and viral microneutralization assays.
Perform high-throughput screening for antiviral drugs and/or vaccine immune correlate studies.
Perform molecular biology techniques, including RNA isolation, molecular cloning, and PCR
Perform cell culture, viral stock, and vaccine stock production.
Perform work in biosafety level 2 and 3 (BSL-2 and BSL-3) virology laboratory, including use of a personal air purifying respirator (PPAR).
Participation in the NIH Biosurety and Select Agent personnel programs.
Perform single cell RNA sequencing (scRNA-seq) using the 10x Genomics platform, including preparing single-cell suspensions.
Provide upkeep and routine maintenance for project, maintaining laboratory supplies and equipment including chemicals, biological materials, etc., required to handle biological materials, recombinant protein materials, acids, biological wastes, etc., maintaining work bench and equipment related to projects.
Maintain detailed records of experiments and their results and prepare the information resulting from these experiments for presentations at laboratory-wide meetings and for publication.
Write reports of findings, stating methods and procedures, including any modifications employed, samples and materials involved and results of experiments.
Interpreting, evaluating, and discussing the results of each experiment with the supervisor as part of the planning process for subsequent studies.
Operate and communicate effectively in a group, team-oriented working environment.
Work with and support others in a collegial, open, respectful, and interactive working manner.
Work collaboratively with others in the lab to generate data for publications.