Biological Scientist II
About The Position
The Biological Scientist II will perform research in the laboratory of Dr. Barry Byrne, MD, PhD in the Department of Pediatrics - Child Health Research Institute (CHRI). Duties will include: Animal Studies: Handling mice for setting up timed matings, weaning animals and maintaining rodent colonies. Will participate in treating animals with pharmacological agents or AAV vectors either through i.p., i.m., or i.v. injection. Other duties with the mice may include doing echocardiography, sensory and motor function, force mechanics, and metabolic studies. Collecting organs and other tissue and embedding the specimens for sectioning or for molecular analyses. Molecular techniques and analysis: Molecular cloning, Plasmid purification, Primer design, RNA and DNA isolation, isolating RNA and performing real-time PCR, and PCR genotyping of transgenic mouse lines. Participate in in vitro studies exploring novel vectors in various cells. This may include expansion/maintenance of cell lines and analysis of transfection/infection. Performing protein analysis by isolating protein of interest from cell or tissue lysates and performing/analyzing western blots. Project Management and Data Analysis: Coordinate the ordering of supplies in advance of experimental need, assist in supervising or directing the work of laboratory personnel, assist in teaching or advising work of laboratory personnel in laboratory methods and/or procedures, assist in directing/coordinating/organizing laboratory activities; upholding laboratory safety policy compliance of all members working in the laboratory, assist with regulatory compliance (EHS, IACUC, etc). Compiling experimental data for presentation in various formats (GraphPad, Excel, etc). Performing various tasks as assigned.
Requirements
- Bachelor’s degree in biology or a related field and three years of experience or Master’s degree in biology or a related field with one year of relevant experience.
Nice To Haves
- Extremely knowledgeable in laboratory procedures and must be meticulous in recording, tracking & reporting of data collected.
- B.S. degree in related scientific field. Masters preferred.
- Doctoral degree in biology or a closely related field; or a master's degree in biology or a closely related field and one year of relevant experience; or a bachelor's degree in biology or a closely related field and three years of relevant experience.
Responsibilities
- Handling mice for setting up timed matings, weaning animals and maintaining rodent colonies.
- Participate in treating animals with pharmacological agents or AAV vectors either through i.p., i.m., or i.v. injection.
- Doing echocardiography, sensory and motor function, force mechanics, and metabolic studies.
- Collecting organs and other tissue and embedding the specimens for sectioning or for molecular analyses.
- Molecular cloning
- Plasmid purification
- Primer design
- RNA and DNA isolation
- Isolating RNA and performing real-time PCR, and PCR genotyping of transgenic mouse lines.
- Participate in in vitro studies exploring novel vectors in various cells. This may include expansion/maintenance of cell lines and analysis of transfection/infection.
- Performing protein analysis by isolating protein of interest from cell or tissue lysates and performing/analyzing western blots.
- Coordinate the ordering of supplies in advance of experimental need
- Assist in supervising or directing the work of laboratory personnel
- Assist in teaching or advising work of laboratory personnel in laboratory methods and/or procedures
- Assist in directing/coordinating/organizing laboratory activities
- Upholding laboratory safety policy compliance of all members working in the laboratory
- Assist with regulatory compliance (EHS, IACUC, etc)
- Compiling experimental data for presentation in various formats (GraphPad, Excel, etc).
- Performing various tasks as assigned.
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What This Job Offers
Job Type
Full-time
Career Level
Mid Level
Number of Employees
5,001-10,000 employees
