Associate Scientist

Regeneron Pharmaceuticals • Tarrytown, GA

9d • Onsite

About The Position

Associate Scientist (Tarrytown, NY) Regeneron Pharmaceuticals, Inc. seeks an Associate Scientist (Tarrytown, NY) to conduct laboratory experiments supporting the design, development, and functional validation of gene therapy platforms, gene editing technologies, novel viral vectors, and nucleic acid therapeutics targeting neurological and neurodegenerative diseases. Support technology development for antibody-based strategies to improve targeted drug delivery across the blood brain barrier (BBB), to the CNS and into specific tissues and cell types of interest. Plan and perform experiments to functionally validate novel targeted drugs (siRNAs, ASOs, shRNAs, CRISPR systems, vectorized antibodies or peptides) and viral vectors in in vitro models. Perform cell culture with immortalized cell lines, iPSC-derived cells and primary cultures, and cell-based assays (ICC, flow cytometry). Plan, initiate, and complete molecular biology and biochemistry assays: molecular cloning, PCR, RT-qPCR, RNA sequencing, ELISAs, IP, WB, CE-western. Perform advanced data analysis (Halo, ImageJ, FlowJo, Prism) and troubleshooting under minimal supervision. Keep up-to-date and well-organized records of experimental protocols and results. Communicate and report experimental findings at group and departmental meetings. Survey current and relevant literature to expand scientific and technical knowledge and optimize and develop new assays.

Requirements

• Requires a Bachelor’s degree in Biology, Neuroscience, Clinical Research, or a related field, and 1 year of in vitro lab experience.
• Must have experience with: Neuroscience, molecular tools for CNS targeting, in vitro models of neurodegenerative disorders
• Aseptic technique and culturing various in vitro cell lines such as HEK293T and primary neurons
• Working with iPSC-derived cells with knowledge in differentiation protocols to generate iPSC-derived neurons and other CNS cell types
• Generating lentivirus and leveraging them to generate stable cell lines in vitro
• Cell-based assay techniques, including immunocytochemistry/immunofluorescence (ICC/IF) and flow cytometry
• Molecular biology techniques and biochemical assays, including cloning, sequencing, PCR, RT-qPCR, ELISA, immunoprecipitation, and western blot
• Data analysis using ImageJ, Prism, and sequence alignment tools.

Responsibilities

• Conduct laboratory experiments supporting the design, development, and functional validation of gene therapy platforms, gene editing technologies, novel viral vectors, and nucleic acid therapeutics targeting neurological and neurodegenerative diseases.
• Support technology development for antibody-based strategies to improve targeted drug delivery across the blood brain barrier (BBB), to the CNS and into specific tissues and cell types of interest.
• Plan and perform experiments to functionally validate novel targeted drugs (siRNAs, ASOs, shRNAs, CRISPR systems, vectorized antibodies or peptides) and viral vectors in in vitro models.
• Perform cell culture with immortalized cell lines, iPSC-derived cells and primary cultures, and cell-based assays (ICC, flow cytometry).
• Plan, initiate, and complete molecular biology and biochemistry assays: molecular cloning, PCR, RT-qPCR, RNA sequencing, ELISAs, IP, WB, CE-western.
• Perform advanced data analysis (Halo, ImageJ, FlowJo, Prism) and troubleshooting under minimal supervision.
• Keep up-to-date and well-organized records of experimental protocols and results.
• Communicate and report experimental findings at group and departmental meetings.
• Survey current and relevant literature to expand scientific and technical knowledge and optimize and develop new assays.

Benefits

• comprehensive benefits, which vary by location. In the U.S., benefits may include health and wellness programs (including medical, dental, vision, life, and disability insurance), fitness centers, 401(k) company match, family support benefits, equity awards, annual bonuses, paid time off, and paid leaves (e.g., military and parental leave) for eligible employees at all levels!

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level
• Number of Employees: 5,001-10,000 employees