Associate Scientist - Protein Sciences

Crossbow Therapeutics, Inc. • Cambridge, MA

4d • $105,000 - $130,000 • Onsite

About The Position

Crossbow Therapeutics is seeking a highly motivated and detail-oriented Associate Scientist – Protein Sciences to join our Antibody Development Team in Cambridge, MA. This individual will contribute to the development and characterization of therapeutic antibodies for both therapeutic applications and research initiatives. This role provides the opportunity to work collaboratively with cross-functional teams to produce, purify, and analytically characterize antibodies and fusion proteins. The ideal candidate will possess a solid foundation in biochemistry or a related field, along with hands-on experience in protein analytical techniques, production, and purification. If you are passionate about protein science and eager to make a significant contribution, this role offers a dynamic opportunity to contribute to innovative therapeutic developments.

Requirements

• Bachelor's degree + 6 years with relevant experience, Master's + 3 years in Biochemistry, Molecular Biology, or a related field.
• Demonstrated hands-on expertise in mammalian expression systems, including CHO and HEK293.
• Demonstrated expertise in protein purification, with the ability to independently design and execute purification strategies with minimal supervision; proficiency with ÄKTA chromatography systems is required.
• Proficiency in chromatography techniques relevant to antibody and bispecific protein production, including Protein A affinity chromatography, ion exchange chromatography (IEX), and size exclusion chromatography (SEC); experience with mixed-mode chromatography or HIC is a plus.
• Experience producing complex biologics, such as bispecific antibodies, multispecific proteins, or engineered antibody formats.
• Solid understanding of protein biochemistry and developability considerations, including purity, aggregation, and stability.
• Working knowledge of protein analytical techniques, with a strong interest and aptitude to expand skills in this area.
• Experience using Agilent HPLC systems for protein analytics.
• Ability to work independently and manage experiments end-to-end, including experimental planning, execution, troubleshooting, and documentation.
• Strong organizational and data analysis skills, with attention to detail and reproducibility.
• Effective written and verbal communication skills for cross-functional collaboration.
• This position requires on-site work 5 days per week at our office in Harvard Square, Cambridge, Massachusetts.

Nice To Haves

• Experience with therapeutic antibody developability assessments is a plus.
• Excellent problem-solving skills complemented by keen attention to detail.
• Ability to thrive both independently and collaboratively in a fast-paced environment.
• Flexibility to adapt to changing plans.
• Strong communication and organizational skills to support effective teamwork.

Responsibilities

• Perform production of recombinant monoclonal and bispecific antibodies, and other proteins using mammalian protein expression systems (e.g., CHO, HEK293).
• Implement various chromatography methods (affinity, IEX, SEC, HIC, MMC) for the purification of antibodies and other proteins.
• Conduct comprehensive analyses to assess protein purity and quality using SDS-PAGE, UV/Vis spectroscopy, HPLC tools, and other relevant analytical methods.
• Operate and maintain AKTA FPLC systems and analytical HPLC instruments to ensure efficient workflow.
• Address and resolve technical challenges that arise in protein analytical and purification workflows, ensuring high-quality results.
• Work with the team to develop and implement sophisticated analytical methods (HPLC, BLI, ELISA, etc.) for the characterization of antibodies and other proteins.
• Document and maintain precise records of laboratory activities using an electronic laboratory notebook (Benchling) and communicate findings clearly through written reports and presentations.
• Actively stay informed about advancements in protein analytical, production, and purification technologies to continuously enhance methodologies.
• Provide technical and resourcing support to other functional groups as needed.

Benefits

• We offer a competitive compensation that includes an annual discretionary bonus and equity package, allowing you to have a stake in our long-term success.
• Base salary will be commensurate with experience and in the range of $105,000-$130,000 for an Associate Scientist.
• Take care of your physical and mental health through our subsidized medical, dental and vision insurance programs.
• Enjoy work and life with our flexible paid time off program, annual holidays including a Winter Break and sick time.
• Utilize our tuition reimbursement program to further your education.
• Maximize your future retirement by participating in our 401k program which offers a match of 100% up to the first 4% of earnings employees contribute.

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level