Associate Scientist, Gene Editing and iPSC Engineering

Eurofins • Boston, MA

19d • $37 - $41 • Onsite

About The Position

We are seeking a highly skilled, passionate, and motivated scientist to join our diabetes team. This position will play a key lab-based role in advancing iPSC stem cell engineering and will contribute to the development of the next generation of islet cell therapies. Successful candidates will have experience with gene editing, molecular biology techniques, assay development, and human iPSC cell culture, differentiation, and banking.

Requirements

• B.S. in Stem Cell Biology, Cell Biology, Bioengineering or a related field with 3-4 years relevant industry experience in gene editing and cell therapy. M.S. in Stem Cell Biology, Cell Biology, Bioengineering or related field with 1-2 years relevant industry experience in gene editing and cell therapy.
• Extensive hands-on experience with human iPSC culture, gene editing, and molecular biology techniques.
• Proficiency in CRISPR gene editing and cell line development, including optimization of protocols, familiarity with flow-based sorting.
• Proficiency in molecular biology techniques including construct design, PCR, ddPCR, dPCR, and NGS.
• Hands-on experience with assay development including luciferase assays, ELISA, and flow cytometry.
• Hands-on experience with cell delivery methods of gene editing tools.
• Excellent communication and interpersonal skills, with the ability to work effectively in a team-oriented environment.
• Strong organizational skills and diligent record keeping.

Nice To Haves

• Hands-on experience in pancreatic islet directed differentiation.
• Hands-on experience in transitioning research-grade processes to GMP-grade.
• Familiarity with electronic lab notebook (ELN) documentation.
• Authorization to work in the United States indefinitely without restriction or sponsorship.

Responsibilities

• Enabling and advancing iPSC gene editing, cell line development, and evaluation of differentiated cells.
• Routine iPSC cell culture, passaging, and media making.
• Optimize workflows for iPSC CRISPR based gene-editing, gRNA screening, and delivery.
• Optimize and perform assays for on-/off-target analysis via PCR and NGS workflows.
• Generate clonal gene edited iPSC lines and characterize via flow cytometry, ddPCR, dPCR and qPCR.
• Culture and execute routine directed differentiation of human iPSCs to pancreatic islets performing drug substance assessments such as cell counts, viability, and metabolite assessments.
• Assist scientists in the team to evaluate and design mitigation strategies to ensure genomic stability of the iPSC lines and drug substance.
• Collaborate cross-functionally to support transfer of R&D assays to GMP.
• Maintain accurate and detailed records of all experiments, synthesize results, prepare reports, and present in meetings. Contribute to SOP development.
• Work seamlessly in executing workplans and meeting deadlines.

Benefits

• Excellent full-time benefits including comprehensive medical coverage, dental, and vision options
• Life and disability insurance
• 401(k) with company match
• Paid vacation and holidays
• Yearly goal-based bonus & eligibility for merit-based increases

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level