Shared Resources Specialist II - Mouse Development Lab (MoDeL)

Fred Hutchinson Cancer Center•Seattle, WA

15h•$32 - $48

About The Position

Fred Hutchinson Cancer Center is an independent, nonprofit organization providing adult cancer treatment and groundbreaking research focused on cancer and infectious diseases. Based in Seattle, Fred Hutch is the only National Cancer Institute-designated cancer center in Washington. With a track record of global leadership in bone marrow transplantation, HIV/AIDS prevention, immunotherapy and COVID-19 vaccines, Fred Hutch has earned a reputation as one of the world’s leading cancer, infectious disease and biomedical research centers. Fred Hutch operates eight clinical care sites that provide medical oncology, infusion, radiation, proton therapy and related services, and network affiliations with hospitals in five states. Together, our fully integrated research and clinical care teams seek to discover new cures to the world’s deadliest diseases and make life beyond cancer a reality. At Fred Hutch we value collaboration, compassion, determination, excellence, innovation, integrity and respect. Our mission is directly tied to the humanity, dignity and inherent value of each employee, patient, community member and supporter. Our commitment to learning across our differences and similarities make us stronger. We seek employees who bring different and innovative ways of seeing the world and solving problems. The Mouse Development Lab (MoDeL) at Fred Hutch is dedicated to advancing mouse model development to drive groundbreaking discoveries in fundamental biology and human disease research. We are seeking a Shared Resources Specialist II- MoDeL , responsible for supporting and collaboratively enhancing the preclinical research needs of researchers using transgenic and preclinical mouse models, at Fred Hutch. We are expanding our team and looking for a genome engineer to help design and refine in vivo models using cutting-edge molecular and cellular biology tools. If you are passionate about in vivo modeling and CRISPR-based genome editing, and enjoy both the molecular and hands-on aspects of mouse research, we encourage you to apply. The Shared Resources Specialist II - MoDeL will perform a variety of technical procedures related to preclinical models and bench top work to support the science of creating and maintaining preclinical mouse models. This position assumes mastery of all requirements and duties of the Specialist I. At Fred Hutchinson Cancer Center, all employees are expected to demonstrate a commitment to our values of collaboration, compassion, determination, excellence, innovation, integrity, and respect. Please include a cover letter describing your interest in this position.

Requirements

Bachelor’s Degree and 2+ years of experience, OR
Associate Degree and 5+ years of experience, OR
GED/High School Diploma and 7+ years of experience.
Familiarity with CRISPR techniques, including design, execution, and validation of genome editing experiments
Experience designing and validating PCR assays for various applications, such as genotyping, cloning, or expression analysis
Hands-on experience in animal care, including mouse colony management, breeding strategies, tissue biopsies for genotyping, and injections through intravenous, intraperitoneal and subcutaneous routes

Nice To Haves

AALAS certification at the LAT or higher level is recommended, and persons hired for this position should continue to pursue this certification as soon as possible once eligible until achieved
Demonstrated ability to learn and adapt to new systems and techniques
Ability to learn all PCM procedural techniques
Strong understanding of preclinical research using in vivo models
Advanced rodent handling and procedural/ surgical skills
Small animal surgeries using stereomicroscope
Understanding of molecular biology techniques and concepts
Experience with bioinformatics tools including but not limited to databases such as GenBank, Ensembl, and UCSC Genome Browser, Sequence alignment tools (e.g., BLAST, ClustalW) and protein Translation and basic sequence analysis
Proficiency in DNA extraction techniques and experience with Sanger sequencing workflows, including sample preparation and data analysis
Experience working in a core lab environment
Excellent communication and organizational skills
Ability to work in a collaborative team-oriented environment
Experience with MS Office suite of applications (Outlook, Word, Excel, Sharepoint, Teams, One Drive, etc)
Record keeping and database management
Capable of undertaking work with minimal supervision and ability to manage multiple projects simultaneously

Responsibilities

Perform basic molecular biology procedures such as designing and validating PCR assays and performing DNA extraction for Sanger sequencing or NGS workflows.
Familiarity with CRISPR techniques, including design, execution, and validation of genome editing experiments is required.
Experience designing and validating PCR assays for various applications, such as genotyping, cloning, or expression analysis is required.
Hands-on experience in animal care, including mouse colony management, breeding strategies, tissue biopsies for genotyping, and injections through intravenous, intraperitoneal and subcutaneous routes is required.
Additional research support tasks may include managing surgical mouse models.
This position is expected to provide a high level of support for users by establishing and maintaining positive, collaborative working relationships with faculty and external users.
Develop advanced protocols or creative solutions and deliver high quality models with an added ability to guide users in model choice.
Be capable of assisting or leading in experimental design collaboratively with users.
Oversee supply management and preparation.
Develop techniques to review and identify new equipment needs.
Develop team SOPs, training materials and guidelines for investigator use.
May supervise work-study students, interns and lab aids.
Present on new or expanded technologies within MoDeL.
Mentor junior staff within the MoDeL.
Report results to users; accurately document laboratory activities; coordinate and initiate equipment maintenance visits from vendors; assist MoDeL Administrative Manager in performing billing and cost accounting; foster positive relationships with vendors and service engineers who support MoDeL.
Assure that all work done is in accordance with an IACUC approved protocol.
Assist investigative staff in the creation of IACUC protocols for use in preclinical models.
Other duties as assigned include work on weekends, evenings, or holiday shifts as part of a rotation.