SENIOR SCIENTIST, STRAIN ENGINEERING

Sutro Biopharma • South San Francisco, CA

22h

About The Position

Sutro Biopharma, Inc. is looking for an energetic and talented individual to join the multidisciplinary Protein Biochemistry group within the Research Department and drive the research activities of the strain engineering program. You will be working to understand and improve the performance of a novel prokaryotic system for biosynthesis of secreted mammalian proteins by designing and executing experiments on protein expression, downstream processing and analysis. Responsibilities extend from gene design through strain characterization, pilot scale protein expression and processing and analysis of expressed protein product. The candidate will design & validate protein expression appropriate to the scale, number-of-samples, and required purity level for the given project, and will implement appropriate assays to characterize product and to better understand the technology and process. In addition, the successful candidate will be required to interface with external collaborators, make presentations to internal and external collaborators and senior management, and to author internal and external publications.

Requirements

• Ph.D. in Biochemistry or a related discipline with at least 5 year’s experience
• Hands-on experience working with small-scale bioreactors is required
• Experience optimizing and evaluating prokaryotic protein expression strains using plasmid transformation, lambda-red recombination and shake flask or bioreactor overexpression preferred
• Experience working with in vivo protein expression required. Experience working with antibodies or antibody fragments preferred
• Familiarity with molecular biology techniques including cloning, PCR, gene knock-ins and knock-outs
• Proficiency with common protein analytical techniques including SDS-PAGE, Western blotting and ELISA
• Familiarity with statistical analysis software for design of experiments, such as JMP, and sequence analysis software, such as SnapGene

Responsibilities

• Help develop Sutro’s prokaryotic protein expression platforms
• Molecular biology including cloning, PCR, generating gene knock-ins and knock-outs
• Screening of new strains for growth, protein expression and product quality in shake flasks
• Evaluate strain performance in small-scale bioreactors through fed-batch or continuous fermentation
• Conduct mid-stream processing including harvest, cell lysis, clarification, and filtration
• Strain banking activities including Development Cell Bank production
• Coordinate early stage E. coli product testing

What This Job Offers

• Job Type: Full-time
• Career Level: Mid Level
• Education Level: Ph.D. or professional degree
• Number of Employees: 101-250 employees