Senior Research Associate, Protein Science

Flagship Pioneering•Cambridge, MA

7d•$81,000 - $107,250

About The Position

We are seeking a highly motivated Senior Research Associate with experience in recombinant protein expression, purification, and characterization to support the development of FL110’s next-generation protein sequencing platforms and products. In this role, you will design, execute, and optimize protein production workflows from construct design through biochemical and biophysical characterization. You will work in a collaborative, interdisciplinary environment where teams rapidly interpret and respond to experimental results to guide platform development. The ideal candidate is an organized and creative experimentalist who enjoys troubleshooting challenging protein targets and contributing to new technologies.

Requirements

BS or MS degree in Biochemistry, Molecular Biology, or a related field, with 4+ years of research experience in an academic or industry laboratory.
Extensive hands-on experience in bacterial recombinant protein expression and purification, spanning the full workflow from DNA construct design and transformation through chromatographic purification using affinity, ion-exchange, and size-exclusion techniques.
Proficiency with ÄKTA purification systems, including designing and executing protein purification methods.
Familiarity with analytical techniques used to assess recombinant protein quality.
Strong organizational skills and the ability to document experimental work in an ELN and maintain records in an electronic LIMS.

Nice To Haves

Ability to independently design, execute, troubleshoot, and interpret experiments related to protein expression, purification, and stability.
Hands-on work with nanopore-forming proteins or related membrane protein systems.
Familiarity with automation in a biochemistry setting.
Experience developing functional assays to evaluate protein activity or performance.
A track record of improving protein yield through construct and protocol optimization.

Responsibilities

Design, express, purify, and characterize soluble and membrane proteins.
Develop and optimize expression and purification strategies for challenging protein targets.
Design and evaluate new protein constructs and variants informed by experimental results.
Characterize proteins using biochemical and biophysical methods to assess purity, stability, and function.
Collaborate with experimental and computational teams to interpret results and guide future protein and nanopore designs.
Document experimental workflows and contribute to shared lab knowledge and technical documentation.