Senior/Principal Associate Scientist, Platform Research

City Therapeutics•Cambridge, MA

51d•$94,000 - $122,000•Onsite

About The Position

We are seeking a highly motivated, detail-oriented Senior/Principal Associate Scientist to join our Platform Research team and contribute to the development of next-generation of RNAi therapeutics. The ideal candidate is passionate about early discovery science, thrives in a dynamic environment, and is eager to learn and adapt as new directions emerge.

Requirements

BS/MS in Biology or a related discipline.
Minimum of 3-5 years of relevant laboratory experience, preferably in a biotechnology environment.
Experience with molecular biology techniques including RT-qPCR.
Experience with In vivo sample processing, in vitro cell culture and assay execution
Detail-oriented with a strong focus on hands-on laboratory work.
Experienced in using electronic lab notebooks (ELNs) for data documentation and management.
Fast learner with a genuine passion for early-stage discovery research.
Adaptable and resourceful, with the ability to manage urgent tasks and shifting priorities effectively.
Experience working in cross-functional teams and collaborating effectively with colleagues across diverse areas of expertise
Please note this role is required to be on-site at our offices in Cambridge, MA (5 days a week).

Nice To Haves

Familiarity with siRNA or RNA-based therapeutics
Experience with biophysical binding assays

Responsibilities

Tissue Processing and Analysis: Perform tissue processing and downstream analyses, including tissue homogenization, lysate preparation, and evaluation of siRNA distribution and efficacy across animal tissues.
In vitro and cell culture assays: Culture and expand immortalized and primary cell lines Perform transfections and in vitro assays to evaluate changes in target gene expression, with demonstrated proficiency in RT-qPCR techniques.
Molecular biology and analytical assays: Execute molecular and biochemical techniques, including RT-qPCR, SL-qPCR, ELISA, and MSD assays, to support platform development and research initiatives.
Assay development: Design, optimize, and implement new assays as needed to advance platform and project objectives.
Data management and documentation: Maintain accurate, organized experimental records and reagent inventories in electronic lab notebooks (ELNs) on a regular basis.