Scientist

Universities of Wisconsin•Madison, WI

34d•$65,000•Onsite

About The Position

The Engin Lab in the Department of Biomolecular Chemistry is looking for a Scientist to contribute to our focus in understanding the mechanism of type 1 diabetes. The Engin Laboratory is focused on understanding how cellular stress response and organelle dysfunction plays a role in beta cell physiology and pathology. Current projects involve studying molecular mechanisms of cellular stress responses in pancreatic beta cells in autoimmune diabetes, ER-mitochondria communication, cellular senescence and the crosstalk between stressed beta cells and immune cells. The work involves working with the beta cell or immune cell-specific mouse models of the stress response genes and mechanistic studies using cell culture (primary mouse and human islets), biochemical and immunological assays (Engin et al., Sci Transl Med 2013, Lee et al, Cell Metab 2020, Chen, et al., Nat Comm 2022). More information about our research and publications can be found at enginlab.org. The Engin Lab offers training on grant writing, supports lab members to attend national meetings and disseminate their science. Candidate will be expected to: Design and conduct experimental work using state of the art technologies, both in vitro and in vivo. Perform data analysis and interpretation of results as required. Participate in multidisciplinary research collaborations. Write technical documents for fellowships and grants and craft manuscripts for publication. Present data in group meetings, seminars and conferences. Assist with training of junior scientists, students and technicians in the lab Contribute to shared lab duties Candidates who demonstrate the following knowledge, skills and abilities will be given first consideration Molecular biology, RNA, protein work and cloning At least three years of experience with mammalian cell culture. Gene manipulation including adeno or lentiviral transduction, siRNA or CRISPR-mediated knockdown. Flow cytometry (complex panel with more than 8 colors) Confocal imaging of cells and tissues. Prior knowledge of cell stress or senescence Applicants for this position will be considered for Scientist I and Scientist II titles. The title is determined by the experience and qualifications of the finalist.

Requirements

At least 3 years of post-doctoral experience in an academic environment with mammalian cell culture and gene manipulation in cells (overexpression via adeno, lenti or retro virus, gene knockdown via si/shRNA and/or CRISPR).
Experience in cloning and molecular biology techniques, RNA extraction, qPCR, and western blot.
Expertise in molecular and cell biology
Ph.D. is required; focus in molecular biology, cell biology, immunology, biochemistry, or related field.

Nice To Haves

Experience with mouse models
Experience with mouse or human islets
Application of drugs to mice and tissue collection
Confocal imaging experience
Research experience on cell stress, senescence, immunology or organelle biology

Responsibilities

Attends and assists with the facilitation of scholarly events and presentations in support of continued professional development and the dissemination of research information
Collects and analyzes research data, conducts experiments and interviews, and documents results according to established policies and procedures under general supervision and limited responsibility
Writes or assists in developing grant applications and proposals to secure research funding
Conducts literature reviews, prepares reports and materials, and disseminates information to appropriate entities
Serves as a main point of contact and liaison with internal and external stakeholders providing information and representing the interests of a specialized research area
Assists with the identification of research problems and the development of research methodologies and procedures