Scientist (Flow Cytometry)

Nextonic Solutions LLC•Bethesda, MD

About The Position

Nextonic Solutions is looking to add a driven, flexible, organized, and meticulous Scientist to our dynamic team. This position shall provide services in support of the overall functions of the Flow Cytometry Core (FCC), Vaccine Research Center (VRC).

Requirements

Ph.D. in Immunology, Cell Biology, Biotechnology, or a related biomedical research field.
Relevant experience in flow cytometry and immunology.
Demonstrated success in developing and implementing cutting-edge flow cytometry techniques.
Experience in providing supervised and unsupervised single cell data analysis solutions for high parameter flow cytometry.
Experience in setting up immunophenotyping pipelines for high-dimensional flow cytometry.

Nice To Haves

Experience in working within a core/shared resource facility preferred.
Strong collaboration and effective communication skills.

Responsibilities

Establish best practices in conducting complex multi-parameter flow cytometry experiments through experimentation and testing of different methods and protocols.
Evaluate and test fluorescent reagents available from commercial vendors and advise on their use.
Identify and implement new flow cytometry-related technologies to enhance VRC’s capabilities in deep profiling immune cells.
Explore and be familiar with analytical tools available to conduct high-dimension data analysis.
Develop protocols for sorting infectious samples, ensuring compliance with international safety standards.
Conduct routine basic and advanced training and educational efforts on flow cytometry methods and applications for researchers and collaborators, including safety and technical workshops for analyzers and cell sorters.
Train staff and users on new equipment and ensure new equipment performs up to the existing standards and tolerances.
Ensure that best practices in flow cytometry are implemented and educate FCC staff and VRC users accordingly.
Work with VRC PIs to develop flow cytometric protocols, data analysis, and data presentation to ensure the highest level of quality.
Support cell sorting projects with well-documented quality procedures and practices.
Engage in risk assessment program for all active cell sorting projects and advise researchers accordingly.
Calibrate and maintain laboratory equipment, troubleshoot issues, and update procedural manuals and methods for the FCC cell sorting facility. Analyze laboratory testing reports and maintain detailed logs of all work-related activities.
Identify and address unexpected test discrepancies, recommending solutions. Interface with researchers, suppliers, reference labs, and equipment personnel to support operational needs.