Scientist, Cell/Molecular Biology

AbbVie•South San Francisco, CA

17h

About The Position

AbbVie's mission is to discover and deliver innovative medicines and solutions that solve serious health issues today and address the medical challenges of tomorrow. We strive to have a remarkable impact on people's lives across several key therapeutic areas – immunology, oncology, neuroscience, and eye care – and products and services in our Allergan Aesthetics portfolio. For more information about AbbVie, please visit us at www.abbvie.com . Follow @abbvie on X , Facebook , Instagram , YouTube , LinkedIn and Tik Tok .

Requirements

Must have a Master's degree in Cellular & Molecular Biology, Cancer Biology, Pharmaceutical Sciences, Pharmacology, or related field & 2 years of experience as a Scientist.
Of experience required, must have 2 years: (i) performing mammalian cell culture in a biosafety level 2 environment; (ii) performing (a) western blotting, immunoprecipitation, WES, or ELISA, (b) DNA/RNA isolation, RT-PCR, separation techniques for primary cells, & (c) gene expression analysis; (iii) handling & processing human tissue samples for biological assays; & (iv) developing, optimizing, & troubleshooting cell-based assays.
Of experience required, must also have 1 year: (i) manipulating gene expression by CRISPR methods in cancer cell types; (ii) utilizing the biological data analysis software tool: GraphPad Prism & at least one of the software tools: Excel, Image Studio, Spotfire, or Compass; & (iii) documenting experimental procedures & methodologies for regulatory filings.

Responsibilities

Execute in vitro or ex vivo experiments to validate novel targets using cell lines & primary human cell sources.
Perform cell culture (suspension & adherent) & assays including but not limited to: DNA/RNA/protein purification, siRNA/CRISPR knockdowns, Lentiviral transfections, immunoprecipitation, WES, western blot, ELISA, MSD, HTRF, quantitative PCR, protein degradation & colorimetric assays.
Collect, analyze, & summarize data for presentations, & reports.
Maintain an up-to-date understanding of new technologies & relevant scientific advances in tumor dependencies & its roles in cancer from literature & scientific meetings.