Research Technologist 1

InternalBellingham, WA
20h

About The Position

In the Lee Lab, the research technologist will use a wide range of established protocols to conduct experiments aimed at understanding the mechanisms and biological impacts of an RNA interference pathway in the single-celled model eukaryote Tetrahymena thermophila. Current projects are focused on identifying the requirements and molecular processes by which the RNA interference pathway protects the genome from DNA damage. These projects will require the use of sterile technique for culturing Tetrahymena and bacterial cells and a variety of molecular biology, biochemistry, genomics/bioinformatics, and microscopy techniques. These techniques may include: reverse transcription-polymerase chain reaction (RT-PCR), PCR/qPCR, recombinant DNA construction/analysis, Western blots, ChIP-seq/RNA-seq, affinity purifications of protein complexes, mass spec proteomics, and widefield and confocal fluorescence microscopy. Additionally, the assistant will provide general support to the lab as needed, which may include: assisting in training new undergraduate and Masters’ students in routine techniques, tracking and receiving orders, maintaining reagent and cell strain inventories, maintaining cell stocks, preparing common reagents and consumables, updating the lab website, monitoring lab equipment and waste needs, and contributing to plastic/glassware cleaning and sterilization. This is a project-employment position ending May 31, 2027, with potential of extension through 2028, pending funding.

Requirements

  • A Bachelor’s Degree in biology, biochemistry, or related field field of science or technology; OR Two years experience as a laboratory technician, research aide, or equivalent
  • Hands-on experience with one or more experimental techniques listed in the Position Responsibilities
  • Strong organizational and written and interpersonal communication skills
  • Ability to commit to a minimum of 2 years
  • All employees must comply with our Immunization policies, including Proof of Rubeola Measles Immunity within 60-days of hire.

Nice To Haves

  • At least ~3-months to 1 year full-time research experience (or equivalent) in a biochemistry, molecular biology, or cellular biology laboratory.
  • Experience with sterile technique, cell culturing, and relevant molecular biology, biochemistry, and/or fluorescence light microscopy techniques
  • Experience with Excel, R Programming language, programming in FIJI/ImageJ, bioinformatics/sequence analysis, and/or biostatistics

Responsibilities

  • Conduct experiments aimed at understanding the mechanisms and biological impacts of an RNA interference pathway in the single-celled model eukaryote Tetrahymena thermophila.
  • Use sterile technique for culturing Tetrahymena and bacterial cells.
  • Perform molecular biology, biochemistry, genomics/bioinformatics, and microscopy techniques.
  • Assist in training new undergraduate and Masters’ students in routine techniques.
  • Track and receive orders.
  • Maintain reagent and cell strain inventories.
  • Maintain cell stocks.
  • Prepare common reagents and consumables.
  • Update the lab website.
  • Monitor lab equipment and waste needs.
  • Contribute to plastic/glassware cleaning and sterilization.

Benefits

  • This position is part of bargaining unit PT and is covered by the collective bargaining agreement with Public School Employees of WWU.
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