Research Technician IV

Children's Hospital of Philadelphia•Philadelphia, PA

53d

About The Position

This role will be tracking the response of live cells harboring fluorescent reporters of ER stress, in order to distinguish between the response to misfolded proteins and the response to calcium stress. It also participates in planned research projects by performing research protocols with minimal direction and guidance from the principal investigator, program director or senior scientists. May exercise moderate discretion and judgment in the execution and interpretation of experiments, and of data derived. Preference will be given to candidates with the following experience: Western Blotting (or Immunoblotting) Confocal microscopy (or fluorescence microscopy) Enzyme assays ELISAs Tissue culture Transfection and transduction Viral vectors

Requirements

High School Diploma / GED - Required
At least two (2) years of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience - Required
Advanced understanding of lab procedures, protocols, and equipment.
Intermediate proficiency with lab specific experiments / techniques.
Intermediate scientific analysis skills.
Excellent verbal and written communications skills
Excellent organizational skills
Strong critical thinking / problem-solving skills
Strong analytical skills

Nice To Haves

Bachelor's Degree - Preferred
At least three (3) years of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience - Preferred
Western Blotting (or Immunoblotting)
Confocal microscopy (or fluorescence microscopy)
Enzyme assays
ELISAs
Tissue culture
Transfection and transduction
Viral vectors

Responsibilities

Performs research procedures on experimental samples with minimal supervision
Formulates and performs complex lab experiments and analyzes/ reports the data
Performs tasks that require knowledge of advanced techniques and lab equipment
Performs advanced calculations and maintains appropriate log and lab notebooks
Assists with planning specific research protocols and procedures
Assists in development & troubleshooting of new techniques and ideas
May be required to modify scientific methods and protocols (in conjunction with PI)
Checks others' results for completeness and accuracy
May be responsible for training and mentoring new staff
May assist in the preparation of grants journal articles, posters and oral presentations
May perform routine and non-routine tests / experiments as needed
Maintains office, lab area, reagents, supplies and equipment
Performs routine maintenance on lab equipment
Coordinates schedules of tests & procedures
Attends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniques
Records results, organizes data and maintains lab notebook in an organized manner
Conducts literary searches as required