RESEARCH TECHNICIAN III

Duke University-posted 4 days ago

$23 - $34/Yr

Full-time • Mid Level

Durham, NC

5,001-10,000 employees

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Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations. Be You. Department of Radiation OncologyCollaborating with the Preston Robert Tisch Brain Tumor Center, Department of Neurosurgery, and Duke Cancer Institute About the Lab The Sulman Lab investigates fundamental mechanisms driving cancer development and progression, with the ultimate goal of identifying better treatments for patients. We employ advanced molecular biology approaches, functional genomic screening, single-cell technologies, and our in-house CARPOOL platform for high-throughput drug evaluation. Research Focus Treatment resistance and radiation response Genetic and pharmacologic screens in cellular and organoid models Functional genomics and 3D chromatin profiling to study enhancer function Tissue processing and molecular analysis of patient-derived samples

Maintain an organized and complete lab notebook of experiments
Propagate cells in culture; perform viability and clonogenic assays
Establish and maintain cancer organoid cultures from human specimens
Perform PCR, RT‑PCR, and Western blots on cell lines, tumor tissues, and mouse tissues
Learn and implement advanced techniques: high‑throughput microscopy, confocal microscopy, flow cytometry
Learn and implement next‑generation sequencing library preparation (analysis available for motivated candidates)
Assist with mouse model experiments as needed
Meet regularly with supervisor to plan experiments, troubleshoot, and discuss results
Participate in lab meetings to present results or discuss literature
Assist in recruiting new lab members and orienting them to lab research
Help maintain reagents and equipment (e.g., buffers, ethanol)
Contribute to a collegial laboratory environment
Set up instruments, materials, and apparatus; operate standard laboratory equipment
Participate in laboratory logistics: inspections, ordering, equipment repair

Undergraduate degree completed by start of employment
Prior laboratory research experience
Intellectually passionate and able to display scientific initiative
Work generally requires skills acquired through the completion of a bachelor's degree in botany, biology, zoology, psychology or other science related scientific field program.
Work requires two years of research experience.
A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience.
OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE
Work requires two years of research experience.
A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience.
OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Candidates will gain the most from the experience if they are able to stay for more than 1 year of time, ideally 2 years or more

Your total compensation goes beyond the dollars on your paycheck. Duke provides comprehensive and competitive medical and dental care programs, generous retirement benefits, and a wide array offamily- friendly and cultural programs to eligible team members. Learn more at: https://hr.duke.edu/benefits/

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