About The Position

The laboratory of Dr. Jason Roh, in the Massachusetts General Hospital Cardiovascular Research Center (MGH CVRC), is seeking a full-time Research Technician I for basic molecular biology methods and small animal work. The laboratory investigates models of aging and heart disease, and how they are affected by genetic, pharmacological, and physiological (exercise, pregnancy) interventions. The selected candidate will join an extraordinary group of talented and motivated basic and clinician scientists whose work has direct translational applications and is linked to potential novel therapeutics.

Requirements

  • Bachelor's degree required, preferably in a basic science discipline.
  • Strong academic record and reliability.
  • Motivated to learn about cardiac physiology and related strategies.
  • Excellent judgment and skills in time management, manipulating biologic reagents and equipment, data collection and organization.
  • Thoughtful short- and long-term planning of experiments.
  • Prior experience doing lab work such as tissue culture is highly desirable.
  • Experience in Biostatistical data analysis is preferred but not required.

Responsibilities

  • Perform basic molecular biology techniques involving cell culture, RNA and protein quantification (qPCR and Western analysis), DNA subcloning (expression plasmid construction and mutagenesis), viral vector construction, immunohistochemistry and histological staining.
  • Conduct assays of enzyme activity, data collection and analysis (datasets involving RNA sequencing, protein expression, post-translational modifications).
  • Maintain mouse colonies, perform genotyping, pharmacological and surgical interventions, echocardiography, and exercise testing.
  • Keep work and data collection highly organized.
  • Present data and progress reports at lab meetings and research conferences.
  • Assist in the joint upkeep of the lab.
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