UNIV - Research Specialist I - Department of Regenerative Medicine

Medical University of South Carolina•Charleston, SC

About The Position

We are seeking a candidate who will become part of a team that investigates cardiovascular development and disease. The candidate will work with the PI, other lab personnel including undergraduate researchers, to assist in our ongoing efforts to pursue the origins of cardiac valve and vascular diseases. Since we utilize mouse models, the candidate will be trained to follow established protocols and guidelines when maintaining, genotyping and breeding several genetically altered mouse models. The candidate will perform general molecular biology techniques as well as work together with the lab manager and PI to troubleshoot and establish novel assays that involve primary cells and tissue. This position also involves analysis of data and preparation of data for figures in presentations and manuscripts. Meticulous record keeping is essential to the duties of the candidate.

Requirements

A high school diploma and two years work experience in a clinical or research setting; a bachelor's degree in chemistry, biology, or other natural, life or health care science may be substituted for the two years of required experience.
Ability to perform job functions in an upright position.
Ability to perform job functions in a seated position.
Ability to perform job functions while walking/mobile.
Ability to climb, using step stool.
Ability to work in temperature extremes (32° F to 100° F) when required due to assignments in cold rooms and/or foreign countries.
Ability to work in confined/cramped spaces.
Ability to bend at the waist.
Ability to twist at the waist.
Ability to fully use both hands/arms.
Ability to perform repetitive motions with hands/wrists/elbows and shoulders.
Ability to fully use both legs.
Ability to reach in all directions, stretch, bend, while moving items/materials from both sitting and standing positions.
Ability to maintain good olfactory sensory function/distinction.
Ability to lift materials up to 20 lbs., unassisted.
Ability to lift and carry 4 liter waste material bottles a distance of 30 feet, unassisted.
Ability to lift and carry 25-30 lbs. jugs of liquid a distance of 100 feet, unassisted.
Ability to lift file boxes, from floor level to counter tops, unassisted.
Ability to lift 40 lbs. centrifuge rotors from floor level to height of 40 inches, then down 20 inches into centrifuge drum, unassisted.
Ability to lift 25 lbs. boxes, from floor level to maximum height of 72 inches, unassisted.
Ability to push/pull file boxes from one location to another at floor level.
Ability to maneuver heavy equipment on bench tops, 50 lbs. or greater, unassisted.
Ability to perform gross motor functions with frequent fine motor movements.
Ability to maintain 20/40 vision, corrected, in one eye or with both eyes.
Ability to match or discriminate between colors, normally.
Ability to hear and recognize various alarms emitting from work area systems.
Ability to hear and/or understand conversations.
Ability to work in a darkroom for processing film, etc.
Ability to be qualified physically for respirator use, initially and annually.
Ability to work rotating shifts as required.
Ability to work overtime as required.
Ability to learn and use new processes, tools and equipment as required.

Responsibilities

Maintain mouse colony and perform experimental and routine procedures using established approvals and protocols. Maintain records of mouse colonies in accordance with the protocol guidelines with supervision of the PI.
The candidate will be trained and perform immunohistochemistry and confocal microscopy to analyze cardiovascular anomalies of disease in murine tissues.
Perform basic histological procedures from tissue generated from mouse models of cardiac development and disease.
Routine lab maintenance, ordering and organization.
Analyze experimental results using appropriate software including excel and photoshop.

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