Research Laboratory Technician I
About The Position
A Research Technician I position is available in the lab of Dr. Christopher Mount in the MGH Pathology Department and Krantz Family Center for Cancer Research. This position requires an individual who will work independently under supervision to generate and evaluate cellular immunotherapies in brain tumor model systems. With training and supervision, this individual will also perform molecular characterization assays including single cell sequencing, spatial transcriptomics, and multiplexed immunofluorescence. The lab develops and evaluates cutting-edge cell immunotherapies in model systems and patient samples at the interface of fundamental biology and cellular engineering with the ultimate goal of developing translatable insights to improve outcomes for brain tumor patients. In addition to the opportunity to contribute to this mission, this role offers the chance to present at laboratory meetings and departmental seminars, and dedicated prior technicians have presented their work at national scientific conferences and co-authored publications. Enthusiasm, work ethic and eagerness to contribute as part of a larger team are valued over specific skills. Candidates are expected to continue in this position for at least two years. The role is a great opportunity for those looking to develop as a scientist and prepare for graduate or medical school. Alternatively, those interested in a longer-term commitment will find opportunities to advance in scientific or management roles within our team.
Requirements
- Undergraduate experience in an academic or industry biosciences laboratory setting are required (with 2 references)
- An ability to clearly articulate one’s role(s) in this past experience, including scientific question or goal, and how one’s work contributed to the efforts of the larger team
- Enthusiasm, eagerness to learn and develop as part of a collaborative team
- Ability to work independently and rigorously document one’s work
- Excellent written and verbal communication skills
- Strong organizational skills and attention to detail
Nice To Haves
- Experience with CAR-T cell production and relevant in vitro assays
- Experience with molecular cloning, including validation of generated constructs
- Prior mammalian cell culture work, including lentiviral production
- Previous work performing single cell sequencing, spatial transcriptomics, or immunofluorescence microscopy
- Experience with histology, including specimen preparation, cryosectioning or FFPE sectioning, and immunostaining
- Software: Microsoft suite including Excel, Graphpad Prism, Flowjo, Snapgene or other molecular cloning software
Responsibilities
- Generate and maintain CAR-T cells, including production of viral stocks, collection of primary T cells, cell transduction and expansion, and maintenance of associated records
- Cell culture of diverse human cell lines, including brain tumor models and patient-derived organoids
- Assay the activity of CAR-T cells in vitro, including cytokine generation, cytotoxicity, and cellular imaging
- Flow cytometric analysis of CAR-T cells
- Molecular cloning, including coordination and assessment of sequencing results and maintenance of documented records of constructs
- Execution of assays including single cell sequencing, spatial transcriptomics, and spatial proteomics
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What This Job Offers
Job Type
Full-time
Career Level
Entry Level
Education Level
No Education Listed
Number of Employees
5,001-10,000 employees
