Research Lab Tech I RESEARCH NEUROLOGY HV

Mass General Brigham•Boston, MA

1d•Onsite

About The Position

The Venkatesh Lab at The Brigham and Women's Hospital, Inc. Mass General Brigham, is seeking a highly motivated lab technician to join their pioneering research efforts in cancer neuroscience. The lab's research focuses on the intersection of cancer biology and neuroscience, specifically characterizing and therapeutically targeting electrical components of disease pathophysiologies. By improving the understanding of cellular and molecular mechanisms involved in neuron-cancer communication and circuit formation, the Venkatesh Lab aims to harness systems-level microenvironmental dependencies of tumor growth to develop innovative therapeutic treatments. The Research Lab Tech I will be responsible for maintaining laboratory equipment, organizing and preparing samples, conducting experiments, and recording and analyzing data.

Requirements

Bachelor's Degree in Biology or a related life sciences major.
1-2 years of laboratory experience outside of academic coursework.
Strong theoretical and practical knowledge of laboratory techniques and procedures.
Familiarity with common laboratory equipment and instruments used in research experiments.
Proficiency in data collection, analysis, and scientific software tools.
Excellent attention to detail, organizational skills, and the ability to multitask and prioritize tasks effectively.
Strong problem-solving skills and the ability to troubleshoot technical issues.
Excellent written and verbal communication skills to effectively convey experimental procedures, results, and challenges.
Demonstrated experience with tissue culture and aseptic technique.
Experience with rodent handling, husbandry, and animal experiments.
Demonstrated experience in molecular techniques, particularly cloning and transfection.
Ability to design, execute, and troubleshoot protocols and experiments for various projects in the lab.
Ability to maintain detailed and accurate laboratory notebooks documenting experimental procedures, results, and observations.
Ability to compile, analyze, and present data in a timely fashion.

Responsibilities

Perform laboratory experiments following established protocols and procedures.
Prepare and handle samples, reagents, and solutions, ensuring accuracy and adherence to safety guidelines.
Operate and maintain laboratory equipment and instruments, conducting routine calibration and troubleshooting as needed.
Record experimental data accurately and maintain organized records of procedures, protocols, and results.
Compile and analyze data using appropriate statistical and analytical methods.
Assist in data interpretation and presentation, contributing to research findings and reports.
Operate specialized instruments such as sliding microtomes, vibrotomes, and cryostats to prepare tissue sections for various organs.
Perform routine cell culture techniques, including cell passage, freezing, and thawing.
Analyze and quantify microscopy results to support project goals and reporting.
Troubleshoot and perform molecular biology techniques such as virus production, primer design, molecular cloning, and transfection.
Perform techniques such as Western blotting, viral transduction, and next-generation sequencing (NGS).
Assist in microscopy imaging, including confocal and fluorescence microscopy.
Learn, master, and implement surgical techniques commonly used in preclinical models.
Optimize and troubleshoot immunofluorescence, immunohistochemistry, and in situ hybridization protocols.
Responsible for the maintenance and care of laboratory mice including breeding, weaning, ear tagging, and allocation to lab members.
Maintain up to date mouse records and reports on mouse census to supervisors.
Perform necropsy and tissue harvesting from various mouse models.
Maintain a clean and well-organized work environment, ensuring proper storage of reagents, cells, and samples.
Manage laboratory inventories, including ordering reagents and maintaining stock levels of commonly used consumables.
Receive, store, and document reagents/packages.
Assist in ensuring compliance with institutional biosafety, animal care, and chemical safety regulations.
Help maintain accurate lab documentation, including protocols, experimental records, and safety data sheets.