Research Associate

University of Colorado•Aurora, CO

1d•Onsite

About The Position

The lab of Dr. Ally Nguyen in the Department of Cell and Developmental Biology at the University of Colorado School of Medicine is seeking a Senior Laboratory Scientist to join our team. This is a senior-level position for a PhD-holding scientist who will serve as a key leader in the lab, maintaining independent research directions within the broader theme of genome regulation while also contributing to the overall management and mentorship mission of the lab. Our lab investigates how the requirements for proteins involved in core cellular processes can differ significantly across cellular contexts, including across cell types, within disease states, and across species. We are focused on identifying selectively required genes, particularly those involved in cell division and the maintenance of genomic integrity and leveraging these differences as a key to unraveling their function. We are also driven to uncover these selectively required genes because they serve as attractive candidates for targeted therapeutics, genes that could act as an “Achilles heel” for cancer cells while minimizing off-target effects on healthy tissues. To approach this, we use targeted and large-scale CRISPR/Cas9-based gene editing approaches in mammalian cell lines, combined with cell biological and molecular techniques. The successful candidate will be expected to develop and lead independent research projects within the lab’s focus on genome regulation. In addition to their own research, this individual will play a critical role in the day-to-day management of the lab, including overseeing lab operations, and mentoring junior trainees such as graduate students, postdoctoral fellows, and research assistants. We value a collaborative, team-based approach to science, and the Senior Laboratory Scientist will be expected to foster that environment.

Requirements

PhD in cell biology, molecular biology, genetics, biochemistry, or a related field
Demonstrated research experience in genome regulation, genome integrity, or a closely related area
Strong track record of independent research, including publications in peer-reviewed journals
Experience with mammalian cell culture and molecular biology techniques

Nice To Haves

Prior experience with CRISPR/Cas9-based gene editing and screening approaches
Expertise in high-resolution microscopy
Experience mentoring or supervising junior researchers
Experience with lab management, including budgeting, ordering, and equipment oversight
Familiarity with viral vector-based transduction systems
Excellent organizational, communication, and interpersonal skills

Responsibilities

Develop and maintain independent research directions in genome regulation within the broader scope of the lab
Design and execute experiments using mammalian cell lines, including embryonic cell types
Perform and optimize CRISPR/Cas9 gene knockout screens and targeted gene editing
Conduct high-resolution microscopy experiments
Carry out plasmid design, molecular cloning, and transduction experiments using viral vectors
Analyze and interpret data, and contribute to manuscript preparation and grant writing
Oversee general lab operations, including purchasing, inventory management, and equipment maintenance
Coordinate onboarding and training of new lab members
Ensure compliance with safety protocols and institutional regulations
Help manage lab budget and resource allocation in coordination with Dr. Nguyen
Mentor and provide day-to-day guidance to junior trainees, including graduate students, postdocs, and research assistants
Assist trainees with experimental design, troubleshooting, and data interpretation
Foster a collaborative and supportive lab culture