Research Assistant I - Laboratory of Dr. Joe Ecker

Salk Institute Office of Technology Development•San Diego, CA

4h•Onsite

About The Position

The Research Assistant I will join a team focused on using epigenomics to create a single-cell resolution atlas of the human brain through sequencing and imaging. This role involves training in specialized single-cell multiomics techniques, including large-scale generation of methylation sequencing libraries using robotic systems, Cryostat sectioning, fluorescence imaging for spatial transcriptomics (MERSCOPE), nuclei production and sorting, and chromatin conformation evaluation. The position also entails general laboratory maintenance, coordination with collaborators and FACS core for cell sorting, participation in team meetings, and meticulous LIMS and data recording. The Research Assistant I will collaborate closely with lab staff and scientists, providing research support. Strong experimental skills, including sterile technique, molecular cloning, DNA/RNA preparation, gel electrophoresis, genotyping, PCR, root sectioning, and microscopy, are essential. The role demands careful attention to detail and the ability to balance support responsibilities within a collaborative scientific environment.

Requirements

Bachelor's degree in Biological Sciences or related scientific discipline required
A minimum of 2 years of experience in a scientific role is required for consideration of a Bachelor's degree in a different discipline
Computer skills: Microsoft Office
Strong experimental skills
Sterile technique
Molecular cloning
DNA and RNA preparation
Gel electrophoresis
Genotyping
PCR
Root sectioning
Microscopy
Careful attention to detail
Ability to effectively balance research support responsibilities within a collaborative scientific environment

Responsibilities

Perform duties to support experiments on a variety of experiments, using bisulfite sequencing, magnetic bead DNA purification and PCR
Perform tissue sectioning with Cryostat
Fluorescence staining protocols for imaging
Isolate cellular nuclei from brain tissue
Chromatin assays
Sample tracking and scheduling
Coordination with others for sample processing
Updating LIMS as necessary with sample information
Attend weekly lab meetings and assist with problem solving
General lab/equipment upkeep
Perform other related duties as assigned by management
Large-scale generation of methylation sequencing libraries using a robotic pipetting system
Cryostat sectioning and fluorescence imaging for spatial transcriptomics through MERSCOPE
Nuclei production and sorting
Treatment of nuclei to evaluate chromatin conformation
General laboratory maintenance
Coordination with collaborators and FACS core for cell sorting
Participation in regular team meetings to discuss progress
Well-organized LIMS and data recording