Professional Research Assistant, Senior Professional Research Assistant, Research Associate (0.6 FTE)

University of Colorado•Aurora, CO

2d•$29,046 - $50,701•Onsite

About The Position

The Department of Pharmaceutical Sciences is currently seeking a highly motivated and skilled Professional Research Assistant or Research Associate to support Dr. Kristofer Fritz and his Lab in their groundbreaking research in the Department of Pharmaceutical Sciences at the University of Colorado, Anschutz Medical Campus, Aurora, CO. This position’s primary purpose is to conduct research for extramurally funded scientific studies. In particular, the research focus for this position is the study of alcohol-associated liver disease through the use of rodent, cell culture, and translational models. Various proteomic and epigenetic techniques are used to investigate alterations in post-translational modification (PTM) of hepatic proteins, specifically within metabolic and redox-dependent pathways. Specific duties include animal handling, enzyme assays, 1D- & 2D-SDS-PAGE, Western blotting, LC-MS/MS sample preparation and method development. The researcher will aid with day-to-day operations of the laboratory, including management of graduate students and undergraduate researchers. Experience in developing research protocols focused on the quantitative analysis of metabolically derived PTMs is highly desired. Special emphasis is placed on skills in animal handling coupled with therapeutic modalities, bioinformatics, and processing tissue samples for LC-MS/MS analysis.

Requirements

Professional Research Assistant Minimum Qualifications: Bachelor's degree in Biochemistry, Chemistry, or a closely related field
Senior Professional Research Assistant Minimum Qualifications: Master’s degree in Biochemistry, Chemistry, or a closely related field
Research Associate Minimum Qualifications: PhD degree in life sciences or a closely related field
Knowledge of Biochemistry and Molecular Biology
Proteomics and Mass Spectrometry
Data Collection and Analysis
Experimental Design
Basic Science Techniques
Data Processing, Spreadsheet, and Word Processing Computer Skills
Ability to Work Independently
Reasoning Ability
Planning and Organizing Skills
Written/Verbal Communication Skills

Nice To Haves

Experience working with animal handling, Western blotting, HPLC-MS/MS, and enzyme assays
Experience working in a research laboratory setting

Responsibilities

Conducts experiments using various proteomics techniques to investigate alterations in post-translational modification of proteins due to metabolic toxicity. Specific duties include animal handling, enzyme activity assays, 1D SDS-PAGE, Western blotting, immunohistochemistry, and assistance with day-to-day operations of the laboratory. Special emphasis is placed on skills in molecular biology techniques and processing murine tissues for analysis.
Method development in advanced HPLC-MS/MS techniques, particularly for PTM capture and detection (immunoprecipitation and click-chemistry).
Compiles and records observed experimental results, analyzes and interprets data, and documents results used to support manuscripts and grant applications.
Collaborates with the principal investigator and other research staff to review scientific literature and develop experimental protocols.
Keeps up to date on lab related research and technical advancements.