Principal Scientist of Gene EditingNew

Legend Biotech • Piscataway, NJ

394d

About The Position

The Principal Scientist of Gene Editing at Legend Biotech will play a crucial role in the Research and Early Development team, focusing on the discovery and development of novel cell therapies for oncology, immunology, and infectious diseases. This position requires expertise in genome editing and off-target analysis, with responsibilities that include conducting gRNA screening, leading functional assays, and developing NGS workflows. The ideal candidate will thrive in a collaborative environment and manage multiple projects effectively.

Requirements

• Ph. D. in genetics, molecular biology, immunology, oncology or related fields
• 4+ years of experience in the field of genome editing
• 2+ years hands-on experience using next generation CRISPR systems (e.g. Prime or Base editors)
• Strong expertise in immunotherapy and cell therapy preferred
• Strong experience with NGS library preparation and troubleshooting
• Hands on experience with off-target assay development, experience with base editor is preferred
• Extensive experience of NGS sequencing and data analysis
• Experience of immune cell isolation, activation, gene delivery and expansion
• Strong experiences with off-target assay development and analysis
• Deep expertise in analytic RNA or DNA assays to measure and analyze gene editing results
• Experience in utilizing bioinformatics tools and software for the analysis of gene editing readouts
• Experience in gene delivery of mammalian cell and tissue culturing is required
• Strong problem-solving skills
• Excellent verbal and written communication skills
• Ability to operate under minimal supervision and in a team environment

Responsibilities

• Conduct gRNA screening using Base editor in primary immune effector cells to determine the KO efficiency and select best gRNA for product development
• Lead, design and execute functional assays to validate off-target issue in primary immune effector cells after gene editing
• Develop and execute in-house NGS workflows, including library preparation and troubleshooting
• Conduct in vitro functional evaluation of gene edited immune effector cells
• Maintain an accurate and updated laboratory notebook
• Maintain compliance with GLP and EHS protocols
• Prepare and present reports, publications, oral presentations and patent application documents

What This Job Offers

• Job Type: Full-time
• Career Level: Senior
• Industry: Professional, Scientific, and Technical Services
• Education Level: Ph.D. or professional degree