Postdoctoral Scholar Research

University of South Florida • Tampa, FL

7d

About The Position

The project focuses on the cellular and molecular mechanisms underlying early blood–brain barrier (BBB) dysfunction in multiple sclerosis (MS). Using a combination of multicellular culture configurations (endothelial cells, astrocytes, pericytes, encephalitogenic T cells, myeloid cells), molecular biology techniques, transgenic BBB-specific knockout/in, and in vivo pathophysiological analyses, the successful candidate will investigate mechanisms of BBB dysfunction in models of MS. The position will be based on 100% research effort.

Requirements

• Requires a minimum of a Ph.D. or equivalent terminal degree in a relevant discipline.

Nice To Haves

• Strong technical skills and the ability to work independently in core laboratory techniques are essential. These include advanced cell culture, confocal microscopy, immunodetection techniques, qPCR, and molecular biology.
• Expertise in designing and conducting complex animal studies is also crucial, along with skills in tissue harvesting and preparation, while adhering to all relevant ethical and safety protocols.
• In addition to technical skills, the ideal candidate will have a proven track record in designing effective research protocols, analyzing and interpreting complex datasets, and troubleshooting experiments.

Responsibilities

• Perform molecular biology techniques, including subcloning, transformations, mutagenesis, plasmid isolations, and mammalian cell transfections.
• Initiate and maintain (multicellular) cell culture, including transwell and 3D platforms.
• Conduct cell culture experiments, including genetic and pharmacological manipulations.
• Crossbreed and genotype transgenic mice, including general husbandry.
• Perform animal surgeries and procedures, including injections, blood draws, perfusions, and tissue harvests.
• Conduct animal physiology experiments, including permeability and transendothelial migration studies.
• Immunostain and collect immunofluorescence micrographs, including confocal.
• Run flow cytometry experiments.
• Perform data analyses and figure preparations.
• Write abstracts and manuscripts

What This Job Offers

• Job Type: Full-time
• Education Level: Ph.D. or professional degree
• Number of Employees: 5,001-10,000 employees