Post-Doc Research Associate - Sharma Lab

UNC-Chapel Hill • Chapel Hill, NC

6d

About The Position

This is a position for a postdoctoral research associate to assess function of neuroimmune interactions in mucosal immunity. The candidate would conduct experiments using murine models of colitis and colorectal cancer and analyze immune functions using muti-parametric flow cytometry and transcriptional analysis. The candidate would conceptualize and execute experiments, analyze data and write manuscripts towards assessing the function of sympathetic neurons in mucosal immunity.

Requirements

• Experience with mouse genetics and generating/maintaining conditional knockout models
• Competency in in vivo experimental models, particularly colitis and colorectal cancer.
• Expertise in isolating and purifying specific immune cell populations (IECs, IE-T cells, macrophages) from tissues- including intestinal, spleen and MLN
• T cell functional assays including stimulation protocols, cytokine production analysis ELISA and multiplex cytokine quantification
• Tissue explant culture and ex vivo functional assessments
• Advanced flow cytometry skills including multi-parameter analysis (up to 15+ color panels), tetramer staining, and intracellular cytokine detection
• Western blotting for phospho-proteins and signaling pathway analysis
• Proficiency in molecular biology techniques (qRT- PCR , Western blotting, ELISA , multiplex assays)

Nice To Haves

• Advanced imaging skills including immunohistochemistry, immunofluorescence, whole-mount tissue imaging, confocal microscopy, and 3D image reconstruction
• Ability to design complex multi-factorial experiments integrating genetic, pharmacological, and physiological manipulations
• Project management skills to coordinate parallel experimental streams
• Experience with host-pathogen interactions, immune regulation, innate and adaptive immune responses

Responsibilities

• Conceptualize and execute experiments
• Analyze data
• Write manuscripts towards assessing the function of sympathetic neurons in mucosal immunity

What This Job Offers

• Job Type: Full-time
• Education Level: No Education Listed
• Number of Employees: 11-50 employees