Laboratory Technician III

Veterans Medical Research Foundation of Sd•San Diego, CA

11h•Onsite

About The Position

The Laboratory Technician performs essential duties to maintain the lab's operations, including replenishing and restocking supplies, cleaning labware, and ensuring proper conditions in incubators. Under the direction of the Principal Investigators (PIs), the technician will also conduct molecular virology and cell biology laboratory experiments, analyze and present the resulting data, and may have the opportunity to contribute to manuscript and grant application preparation.

Requirements

Bachelor’s degree (BA/BS) in a related field from an accredited college or university AND at least 2 years of related experience; or an equivalent combination of education and experience

Responsibilities

Make common/shared lab solutions and media, e.g., LB broth, for bacteria (once per week)
Transport collected and double-red-bagged biohazardous trash to loading dock for disposal as medical waste, 2-3 times per week
Cleans and autoclaves glass- and plastic-ware, once per week or once every two weeks
Replaces 10% household bleach in buckets, every other day
Stocks/shelved newly received supplies and logs them as received (as needed, maximum daily)
Checks cell culture incubators for water and associated CO2 tanks; assists with replacing tanks when needed (twice per week)
Checks freezers, including liquid nitrogen freezer for ice accumulation, liquid nitrogen level, and compressor air filters for dust
In collaboration with the Pls, conduct experiments involving standard cell biology and molecular virology methods, including PCR, agarose gel electrophoresis, Western blotting, luciferase assays, ELISA, cloning and plasmid preparation, and cell culture including transfection and infection with HIV-1 and lentiviral vectors
Keep detailed and accurate records of experimental procedures and results
Participate in lab meetings and research meetings with collaborators and colleagues
Analyze and interpret data and prepare results for presentation at regular lab meetings
Attend all required training sessions (e.g., lab safety modules) and adhere to due dates for refresher training
Maintain a collegial relationship with lab members and collaborators
Monitor for and report any unsafe laboratory conditions including equipment that is not in compliance with safety standards