Intern- Process Development, DNA Development

Asklepios BioPharmaceutical, Inc. (AskBio) • Durham, NC

3d • Onsite

About The Position

AskBio Inc., a wholly owned and independently operated subsidiary of Bayer AG, is a fully integrated gene therapy company dedicated to developing life-saving medicines and changing lives. The company maintains a portfolio of clinical programs across a range of neuromuscular, central nervous system, cardiovascular, and metabolic disease indications with a clinical-stage pipeline that includes investigational therapeutics for congestive heart failure, limb-girdle muscular dystrophy, multiple system atrophy, Parkinson’s disease, and Pompe disease. AskBio’s gene therapy platform includes Pro10™, an industry-leading proprietary cell line manufacturing process, and an extensive array of capsids and promoters. With global headquarters in Research Triangle Park, North Carolina, and European headquarters in Edinburgh, Scotland, the company has generated hundreds of proprietary capsids and promoters, several of which have entered pre-clinical and clinical testing. Our vision: Pioneering science to create transformative molecular medicines. Our mission: Lead innovative science and drive clinical outcomes to transform people's lives. Our principles: Advance innovative science by pushing boundaries. Bring transformative therapeutics to patients in need. Provide an environment for employees to reach their fullest potential. Our values: Be a Pioneer. We are not afraid of the impossible and to innovate to make gene therapies accessible to those in need. Cultivate Collaboration. Strive to be the best teammate, actively listen, openly communicate, and embrace diverse points of view. Embrace Responsibility. We are humbled by the enormity of our mission. We hold a relentless commitment to advance science and clinical outcomes for our patients, families, and caregivers. Raise the Bar. Continuously drive improvements and efficiencies. Seek and provide constructive feedback. Have a bias for learning and action. Act with Uncompromising Integrity. Be honest, transparent, and committed to doing what’s right in every situation. Make clear commitments and follow through. Position Summary The purpose of the Process Development, DNA Development Intern position is to support the DNA Development team in ongoing development activities. This project will contribute to evaluating the impact of Inverted Terminal Repeats stability on rAAV manufacturing. This role offers hands-on experience in a laboratory and exposure to key elements of molecular biology and plasmid production. This position is based in RTP, NC and will report to the Associate Director, DNA Development.

Requirements

• Must be enrolled in Bachelor’s degree program in a relevant field (Molecular Biology, Biotechnology, Chemistry/Biochemistry, Chemical/Biochemical Engineering, or equivalent)
• Must be at least a junior at university at the time of your application
• Should have a basic understanding of one or more of the following techniques: aseptic technique, making reagents at desired dilutions or concentrations, Restriction Enzyme digest, Gel Electrophoresis, DNA quantitation, identifying annotated features on a plasmid map, bacterial cell culture, centrifugation, filtration, and liquid chromatography
• Strong attention to detail, highly organized and the ability to positively interact in a team environment
• Excellent verbal and written communication skills with the ability to maintain formal record keeping
• Comfortable with working and learning in a fast-paced laboratory environment
• Service-oriented and quality mindset/attitude
• Proficiency in Microsoft Office Suite (Word, Outlook, Excel)

Nice To Haves

• Prior internship, work, or volunteer/extracurricular experience
• Prior experience working in a lab environment
• Experience with SnapGene software

Responsibilities

• Learn & support plasmid constructs generation, including cloning strategy, transformation, colony screening, and establishing bacterial glycerol stocks.
• Perform bacterial culture growth to support & optimize plasmid production process.
• Purify plasmid DNA from bacterial cultures using established laboratory methods.
• Communicate experimental observations and progress regularly with hiring manager and project team.
• Prepare and deliver a final presentation summarizing project outcomes at the end of the internship.
• Operate within the team structure, working under the direct guidance of an assigned mentor and reporting progress to the hiring manager.

What This Job Offers

• Career Level: Intern
• Number of Employees: 501-1,000 employees