BME Stachowiak Lab - Senior Biochemistry Specialist and Lab Manager

University of Texas at Austin

115d • $65,000 - $85,000

About The Position

The Stachowiak Lab at UT Austin advances understanding of the biophysical mechanism responsible for cellular membrane traffic and cytoskeletal remodeling, while also developing novel biomaterials and biomolecular therapeutics. We are seeking a biochemistry specialist to replace a longtime employee who is retiring. The successful candidate will primarily be responsible for expression and purification of recombinant proteins, while also assisting the Principal Investigator and lab members with laboratory organization and documentation.

Requirements

• A bachelor’s degree in biochemistry, biology, or a related field with 4 years of related work experience OR A master's degree in biochemistry, biology, or a related field with 2 years of related work experience.

Nice To Haves

• A master’s degree in these fields is desired, though work experience is more important.
• Substantial experience with recombinant protein expression and purification is essential.
• Experience with fast protein liquid chromatography (FPLC) is desired.
• Experience with DNA cloning and plasmid design is desired.
• Experience with quantitative, gel-based analysis of protein purity is desired.
• Experience working with intrinsically disordered proteins is desired.
• Ability and drive to solve practical problems.
• Strong organizational skills.

Responsibilities

• Expression and purification of recombinant proteins including plasmid preparation, growth of cultures, induction of expression, optimization of induction conditions, evaluation of expression, cell lysis, protein extraction, assessment of protein purity and solubility, protein storage.
• Quantitative evaluation and documentation of protein purity using gel electrophoresis.
• Optimization of protein purification via modification of induction, expression, and purification protocols.
• Following established protocols to achieve high quality protein purification.
• Development of protocols for purification of novel proteins.
• Documentation of laboratory protocols.
• Training of laboratory personnel in protein expression and purification.
• Monitoring laboratory supplies and stocks.
• Assisting lab members with supply orders.
• Maintaining lab safety protocols and providing safety training to new lab members.
• Participation in scientific publications through protocol development and documentation.

Benefits

• Competitive salaries
• Full benefits
• Extensive support network
• Collaborative working community

What This Job Offers

• Job Type: Full-time
• Career Level: Mid Level
• Education Level: Bachelor's degree
• Number of Employees: 251-500 employees