Biologist

Axle•Baltimore, MD

1d•Onsite

About The Position

Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH). Axle is seeking a Part-Time Biologist to join our vibrant team at the National Institutes of Health (NIH) supporting the National Institute on Aging (NIA) located in Baltimore, MD.

Requirements

Bachelor’s degree in Biology, Life Sciences or a related discipline
Field of Study: Biology
MS office suite
Ability to communicate both orally and in writing

Responsibilities

Provide support services to satisfy the overall operational objectives of the National Institute on Aging.
Cultivate, maintain, expand, and freeze ES and iPS cell lines from mouse and human.
Prepare ES and iPS cells for N-linked glycoprotein labeling and analysis and for flow analyses.
Characterize new iPS cell lines.
Investigate state of pluripotency of ES and new iPS cell lines.
Characterize nuclear proteins and cell surface proteins by western blot, immunoprecipitation, immunochemistry, and PCR.
Identify/recommend resolution of discrepancies.
Recommend/determine the appropriate data to use, record or report.
Create/maintain an accurate research tracking system and database for all research activities.
Keep accurate records of daily experiments and results.
Prepare reports, spreadsheets and slides for presentation and/ or publication.
Write and edit scientific papers.
Present ongoing findings at various meetings.
Perform routine maintenance on laboratory instruments and equipment.
Ensure supplies are properly stocked.
Obtain quotes and prepare purchase requests.
Clean Equipment.
Inspect Equipment.
Meet with lab members to present updates.
Program and use computers to store, process, and analyze data.
Interpret research or operational data.
Record research or operational data.
Collect and analyze biological data about relationships among and between organisms and their environment.
Monitor and observe experiments, recording production and test data for evaluation by research personnel.
Provide technical experience needed to assist in studying the basic principles of plant and animal life.
Study basic principles of plant and animal life, such as origin, relationship, development, anatomy, and function.
Identify, classify, and study structure, behavior, ecology, physiology, nutrition, culture, and distribution of plant and animal species.
Assist with performing basic laboratory experiments and procedure.
Analyze experimental data and interpret results to write reports and summaries of findings.
Prepare ES and iPS cells for N-linked glycoprotein labeling and analysis by Mass Spectrometry.
Prepare ES and iPS cells for flow analyses, including the labeling of the cells with appropriate antibodies.
Generate primary cultures of cells for the generation of new iPS cell lines.
Characterize new iPS cell lines using appropriate techniques (morphology, staining, growth traits).
Prepare primary cell cultures suitable for iPS cell formation using standard nucleofection based technique.
Perform primary cell cultures of mitotically inactivated feeder layers.
Design and execute flow cytometry (FACS) preps, proliferation assay, and cell surface receptor analysis.
Design primers as needed for cell characterization.
Apply a wide range of conventional, established approaches, methods, techniques and solutions to new situations.
Prepares, organizes and maintains various lab samples, supplies, and equipment.
Maintain an accurate research tracking system and database for research activities.
Keep accurate records of daily experiments and results including research documentation.
Prepare data, results, reports as well as Excel spreadsheets and PowerPoint slides, for oral presentation and/or publication in journals.
Attend monthly laboratory meetings.
Participate in lab/unit/section meetings by presenting ongoing research and findings.
Present paper/abstract/poster at scientific meeting(s)/conference(s).
Operate and perform routine maintenance on laboratory instruments and equipment.
Ensure scientific supplies are kept stocked.
Obtain quotes and prepare purchase requests for supplies, instruments and equipment, and submit to primary investigator and/or lab manager for approval.
Learns new laboratory techniques and procedures.