Biologist

Guidehouse•Bethesda, MD

1d•$65,000 - $108,000

About The Position

Provide support of lab activities related to the structural biology of viruses and vaccine particles and vaccine development, such as but not limited to: prokaryotic and eukaryotic cell culture techniques for the production and purification of influenza viruses and the production of recombinant protein antigens and antibodies by gradient centrifugation and column chromatography. Perform cell culture techniques related to cell-based assays such as antibody-dependent cellular cytotoxicity (ADCC,) microneutralization, Median Tissue Culture Infectious Dose (TCID50) assays would be preferred. Perform general biochemical and immunological assays such as hemagglutination inhibition assay, SDS-PAGE, Western blot, and ELISA. Perform and analyze immunological assays according to Laboratory Standard Operating Procedures, including but not limited to Western Blot, ELISA, ADCC, microneutralization, and (TCID50) assays, HAI and other inhibition assays. Perform molecular biology/virology methods including but not limited to DNA extraction and purification, PCR, RT-qPCR, molecular cloning, gel electrophoresis, transfections, bacterial transformations, recombinant protein production/purification and microscopy. Maintain cell cultures (e.g., Vero, MDCK cells), and perform virus amplification, harvest, and titration. Receive, organize, and distribute specimens, documenting shipments using specified electronic systems. Maintain cell cultures (e.g., Vero, CRFK, Sf-9, CHO, RAW264.7, CaCo-2 cells, intestinal enteroids), and perform virus or VLP amplification, harvest, and titration.

Requirements

Bachelor’s degree
Minimum of two (2) years of related lab experience.
RNA purification and RT-qPCR experience required.
Strong communications skills, both oral and written.
Apply Good Laboratory Practice (GLP) principles to characterize test articles, analyze specimens, and maintain detailed records of assays and data.
Excellent analytical, organizational and time management skills.
Must be able to OBTAIN and MAINTAIN a Federal or DoD "PUBLIC TRUST"; candidates must obtain approved adjudication of their PUBLIC TRUST prior to onboarding with Guidehouse. Candidates with an ACTIVE PUBLIC TRUST or SUITABILITY are preferred.

Nice To Haves

Degree in Biology

Responsibilities

Provide support of lab activities related to the structural biology of viruses and vaccine particles and vaccine development
Perform prokaryotic and eukaryotic cell culture techniques for the production and purification of influenza viruses and the production of recombinant protein antigens and antibodies by gradient centrifugation and column chromatography.
Perform cell culture techniques related to cell-based assays such as antibody-dependent cellular cytotoxicity (ADCC,) microneutralization, Median Tissue Culture Infectious Dose (TCID50) assays
Perform general biochemical and immunological assays such as hemagglutination inhibition assay, SDS-PAGE, Western blot, and ELISA.
Perform and analyze immunological assays according to Laboratory Standard Operating Procedures, including but not limited to Western Blot, ELISA, ADCC, microneutralization, and (TCID50) assays, HAI and other inhibition assays.
Perform molecular biology/virology methods including but not limited to DNA extraction and purification, PCR, RT-qPCR, molecular cloning, gel electrophoresis, transfections, bacterial transformations, recombinant protein production/purification and microscopy.
Maintain cell cultures (e.g., Vero, MDCK cells), and perform virus amplification, harvest, and titration.
Receive, organize, and distribute specimens, documenting shipments using specified electronic systems.
Maintain cell cultures (e.g., Vero, CRFK, Sf-9, CHO, RAW264.7, CaCo-2 cells, intestinal enteroids), and perform virus or VLP amplification, harvest, and titration.

Benefits

Medical, Rx, Dental & Vision Insurance
Personal and Family Sick Time & Company Paid Holidays
Parental Leave
401(k) Retirement Plan
Group Term Life and Travel Assistance
Voluntary Life and AD&D Insurance
Health Savings Account, Health Care & Dependent Care Flexible Spending Accounts
Transit and Parking Commuter Benefits
Short-Term & Long-Term Disability
Tuition Reimbursement, Personal Development, Certifications & Learning Opportunities
Employee Referral Program
Corporate Sponsored Events & Community Outreach
Care.com annual membership
Employee Assistance Program
Supplemental Benefits via Corestream (Critical Care, Hospital Indemnity, Accident Insurance, Legal Assistance and ID theft protection, etc.)
Position may be eligible for a discretionary variable incentive bonus