Associate Scientist - Molecular Discovery

Pfizer • San Diego, CA

4d • Onsite

About The Position

Pfizer BioMedicine Design group at San Diego, CA is seeking an outstanding and highly motivated Associate Scientist with training in protein biochemistry to join a dynamic team in antibody discovery, optimization, and production. This role requires good understanding and hands-on skills in protein/antibody production and engineering. What You Will Achieve In this role, you will: Work within a team on various antibody identification, optimization, and/or production projects in parallel. Maintain mammalian cell culture and execute various transient expression methods. Implement molecular biology methods such as reformatting scFv to IgG and other modalities. Implement protein purification methods to isolate IgG, fusion proteins and antigens from mammalian cell culture. Analyze proteins using standard protein characterization methods. Design experiments, analyze data, and troubleshoot when issues arise, under supervision. Present experimental design and results at internal meetings. Accomplish goals under project team timelines.

Requirements

• B.S or M.S in Biological Science (Molecular Biology, Structural Biology, Biochemistry, Cell Biology) or a related field (Chemistry, Chemical Engineering, Biomedical Engineering and Biological Engineering)
• B.S or M.S with 0-2 years of relevant experience in either an academic or industrial setting.
• Familiar with tissue-culture sterile techniques and mammalian cell culture.
• Understand principles and able to execute modern molecular biology techniques, preferred in antibody engineering field.
• Understand principles and able to execute modern protein purification techniques, preferred in antibody engineering field.
• Detail-oriented, document data and experiments properly, good communication and interpersonal skills.
• Ability and desire to work in a fast-paced interdisciplinary scientific team and the desire to learn new techniques.
• Strong time management skills and ability to manage multiple projects at the same time.

Nice To Haves

• Previous experience with protein and/or antibody engineering
• Previous experience with suspension mammalian cell culture and transient expression
• Previous experience with molecular cloning techniques (primer design, PCR, gel extraction, restriction/homology cloning), sequence confirmation, and plasmid preparation
• Previous experience with protein purification (protein A/G affinity, IMAC, size exclusion, ion exchange chromatography) and protein characterization methods, including SDS-PAGE/cGE, aSEC, mass spec, endotoxin measurement.

Responsibilities

• Work within a team on various antibody identification, optimization, and/or production projects in parallel.
• Maintain mammalian cell culture and execute various transient expression methods.
• Implement molecular biology methods such as reformatting scFv to IgG and other modalities.
• Implement protein purification methods to isolate IgG, fusion proteins and antigens from mammalian cell culture.
• Analyze proteins using standard protein characterization methods.
• Design experiments, analyze data, and troubleshoot when issues arise, under supervision.
• Present experimental design and results at internal meetings.
• Accomplish goals under project team timelines.

Benefits

• We offer comprehensive and generous benefits and programs to help our colleagues lead healthy lives and to support each of life’s moments. Benefits offered include a 401(k) plan with Pfizer Matching Contributions and an additional Pfizer Retirement Savings Contribution, paid vacation, holiday and personal days, paid caregiver/parental and medical leave, and health benefits to include medical, prescription drug, dental and vision coverage.

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level
• Number of Employees: 5,001-10,000 employees