Sr Scientist In Vitro R&D Contractor

ProKidney • Morrisville, NC

3h

About The Position

We are seeking a highly skilled and motivated Scientist / Senior Scientist to support mechanism of action (MoA) elucidation for rilparencel, our autologous kidney cell therapy currently being evaluated in a Phase 3 clinical study, as well as the development of next-generation cell therapies. Working closely with cross-functional teams, you will translate translational biology hypotheses into rigorous experimental designs that generate the definitive data needed to support publications and potential regulatory filings. The ideal candidate is a strategic scientific problem-solver who navigates the non-linear dynamics of complex biological systems. You possess the scientific maturity to design robust experimental frameworks that de-construct biological noise and contextualize donor variability. Leveraging hands-on expertise in multi-parameter flow cytometry, cell sorting, and genetic engineering, you will design advanced models to deconvolute cellular heterogeneity and interrogate pathways of interest. You possess the ability to synthesize high-dimensional, multi-modal datasets into actionable insights that shape our overarching translational strategy and cross-functional decision-making.

Requirements

• Ph.D. in Cell Biology, Systems Biology, Immunology, Molecular Biology, Bioengineering, or a related field with 0+ / 2+ years of industry or relevant post-doctoral experience; OR a Master’s degree with 2+ / 4+ years of industry experience; OR a Bachelor’s degree with 4+ / 8+ years of industry experience
• Multi-parameter flow cytometry panel design, execution, and data analysis for cellular phenotyping and functional profiling.
• Hands-on experience with molecular gene editing tools to engineer cells or model specific pathway manipulations.
• A proven track record of independently designing, optimizing, and functionally validating non-standard cell-based assays.
• Hands-on experience working with primary human cells, complex co-culture systems, or tissue models where cell behavior is highly context dependent.
• Capability to operate with autonomy in a fast-paced environment, shifting technical directions fluidly as new data emerges.
• Assay development and validation
• Flow cytometry / FACS
• Molecular biology and gene editing
• Mammalian cell culture / co-culture
• Analytical instrument troubleshooting
• Data synthesis and statistical analysis
• SOP compliance
• Analytical problem solving
• Cross-functional collaboration
• Project management and autonomy

Responsibilities

• Design in vitro systems that deliberately account for and characterize donor heterogeneity and functional variability within an in vitro disease model system.
• Utilize multi-parameter flow cytometry (FACS) for high-resolution cell sorting, phenotyping, and functional characterization within heterogeneous populations.
• Apply gene editing technologies to interrogate specific molecular mechanisms.
• Develop and evaluate novel functional testing methodologies to identify and inform prospective therapeutic potency metrics.
• Establish rigorous experimental controls to maximize signal-to-noise ratios and autonomously investigate complex biological datasets to inform experimental strategies.
• Design highly reproducible workflows that ensure data integrity and adherence to standard operating procedures.
• Integrate and visualize multi-modal datasets to generate a cohesive understanding of biological mechanisms.
• Communicate experimental findings effectively across cross-functional groups.

What This Job Offers

• Job Type: Full-time
• Career Level: Senior
• Education Level: Ph.D. or professional degree