Senior Research Associate, Antibody Discovery & Engineering

About the position

We are seeking a Senior Research Associate to support antibody discovery and engineering at Tentarix. The primary responsibilities for this role will be antibody engineering using phage and mammalian display platforms, including DNA library design and cloning, library screening, and biochemical characterization of identified binders and engineered multi-specific biologics. We are seeking an individual who will thrive in a start-up environment and works well across groups in a collaborative environment.

Responsibilities

• Design and construct single and multi-domain protein expression constructs, including plasmid sequencing and scale-up for expression
• Design and generate mammalian and phage display libraries for affinity maturation (previous phage display experience not required)
• Collect and analyze sequencing data from cloned plasmid variants and phage display libraries using in-house NGS instrument (MiSeq i100) and/or Sanger sequencing
• Characterize identified binders by BLI/ELISA, and collaborate with analytics team for additional characterization (aSEC, aHIC, DSF, DLS, LCMS)
• Independently plan and execute experiments, analyze results, and present data at team meetings
• Maintain meticulous research records using Benchling electronic notebook and molecule registry

Requirements

• B.S. or M.S. with 4+ years of industry experience in molecular biology and recombinant protein design and expression
• Experience with plasmid cloning methodologies, including Gibson/In-fusion, Golden Gate assembly, and restriction enzyme digests/ligations
• Technical experience in the development and execution of ELISAs, BLI, and other methods for protein quantification and binding characterization
• Experience with transient transfection and CHO/HEK293 suspension cell culture is desired
• Goal oriented, organized, with strong multi-tasking abilities
• Excellent verbal and written communication skills
• Thrive in a cross functional team environment

Nice-to-haves

• Practical experience using phage display for antibody discovery
• Practical knowledge and experience in antibody engineering
• Proficiency in protein structural analysis using PyMol or related applications
• Experience with Phage Display DNA library design and construction for naïve libraries and affinity maturation
• Proficiency in interpreting and presenting data collected from NGS, Sanger Sequencing, BLI, ELISA, and Flow Cytometry

Benefits

• Attractive compensation and benefits packages
• Bonus target
• Equity