Scientist, Antibody Conjugation

Alnylam Pharmaceuticals•Cambridge, MA

1d•$110,400 - $165,600•Onsite

About The Position

We are seeking a Scientist to join our Protein Sciences group to help with our antibody conjugation efforts. The candidate must be independent, goal oriented, technically competent, and able to work efficiently across multiple projects. We are searching for enthusiastic, innovative, and highly motivated individuals, with a scientific background in antibody-drug conjugates including the assembly, purification, and analysis of these molecules. This position is onsite and will be primarily located in Cambridge, MA.

Requirements

The candidate should have a PhD degree with 0-3 years of relevant experience
Strong understanding of antibody conjugation chemistries with both stochastic and site-specific techniques
Strong understanding of modern ADC chemistry and linker design highly preferred
Hands on experiences with preparing antibody-drug conjugates and analyzing them using multiple methods (IEX, HIC, SEC, mass spec)
0-2 years of pharmaceutical or biotechnology industry experience is preferred
Experience with Agilent and AKTA software
Experience with recombinant protein analysis (affinity, size exclusion, reverse phase, hydrophobic interaction, ion exchange chromatography, SDS-PAGE, and UV-VIS spectroscopy)
Passionate about driving his or her projects to independently contribute to research directions and solve challenging issues
Detail oriented, self-motivated, and comfortable working with highly technical projects
Ability to work on multiple projects simultaneously and deliver conclusive results
Highly adaptable and responsive to new priorities in a fast-paced environment
Effective written and oral communication skills
Experience with ELN record keeping
Team oriented with strong work ethic

Nice To Haves

Understanding of antibody design and cloning is nice to have
Understanding of antibody ligand/target validation using BLI and SPR for kinetic analysis is nice to have
Experience with SPR and/or Octet binding experiments is nice to have

Responsibilities

Perform chemical reactions to assemble antibody-oligonucleotide conjugates using various conjugation handles, linkers, and antibody formats (IgG, Fab, scFv, bispecific)
Utilize multiple strategies for stochastic and site-specific conjugation
Optimize reactions and purification methods to improve yields and efficiency ranging from mg to gram scale
Independently troubleshoot conjugation reactions for improved yields and processes
Analyze conjugates using all relevant techniques (IEX, HIC, SEC, SDS-PAGE, mass spec, UV-Vis)
Drive automation for high-throughput conjugation at various scales
Work closely with biology teams to plan studies and generate materials on time for multiple projects
Proper electronic notebook record keeping with high attention to detail
Communication of experimental plans and data to multiple cross-functional scientific teams
Work closely with biologists, chemists, and pharmacologists to contribute to the strategic direction of research programs across multiple therapeutic modalities
Ability to train and supervise associate scientists in the laboratory
Experience preparing and presenting using PowerPoint software