About the position
Applications are sought for a full-time Assistant Project Scientist position in the Samelson Lab. The successful candidate will combine extensive chemoproteomics experience, protein expression, mammalian tissue culture, spectroscopy, and bioinformatics skills to understand iron and iron-chaperone distribution, function, and disease-relevance in human cell culture models of neurodegenerative diseases. The Samelson Lab is looking to understand how metals, especially those related to electron transport or iron homeostasis, affect disease-relevant phenotypes, such as protein aggregation, mitochondrial dysfunction, DNA damage, and lysosomal dysfunction. We are looking to combine mass-spectrometry-based methods for assessing iron occupancy in vivo with proteome-wide iron-chaperone interactome maps and genetic modifiers of these processes using CRISPR-screening.
Responsibilities
- Combine chemoproteomics experience with protein expression and mammalian tissue culture.
- Utilize spectroscopy and bioinformatics skills to study iron and iron-chaperone distribution.
- Conduct mass-spectrometry-based assessments of iron occupancy in vivo.
- Analyze proteome-wide iron-chaperone interactome maps.
- Collaborate with geneticists, biochemists, and chemical biologists to design and implement experiments.
Requirements
- PhD in Chemistry or Biochemistry related discipline.
- At least 4 years post graduate fellowship or experience.
- Ability to work in a collaborative interdisciplinary environment.
- Deep knowledge of the role of metals in human cells.
- Experience generating and analyzing mass spectrometry data.
- Experience with analysis of protein-metal binding both in vitro and in vivo.
- Experience analyzing large protein-protein interaction datasets.
- Experience with sequencing, cloning, cell culture, DNA/RNA/Protein purification.
- Experience with standard molecular and epigenetic methods.
Nice-to-haves
- Experience studying heavy metal (especially iron) biology.
- Interest in neurodegeneration.
- First-author publication in relevant field.
- Experience in the biology of heavy metals.
- Experience in proteomics.
Benefits
- Salary range: $104,000 to $195,000.
- Review timeline: every 3 years from initial appointment.
